Proteomic datasets from HEK293 and braintumor cells are accessible via the surface protein atlas (https://wlab.ethz.ch/cspa/). Proteomic datasets of JK-6L cells are currently unpublished proprietary data available upon request. The relative gene expression data are included in the supplementary ...
Acta Pharmacologica Sinica npg 94 www.nature.com/aps D'Onofrio PM et al While necrosis is more dominant in the ischemic core, apop- tosis becomes more common in the penumbra as the cells are found further away from the core[2]. Apoptosis is a normal process during development, and is ...
利用CRISPR/Cas9技术构建敲除MEIS2基因的HEK293T人胚肾细胞系 CRISPR/Cas9系统作为一种新型的基因组编辑技术,利用人工设计的向导RNA(singleguide RNA,sg RNA)介导外源表达的Cas9蛋白与基因组靶点特异性结合以实现对基因组DNA的特... 卢利莎,白杨,刘鑫,... - 《中国细胞生物学学报》 被引量: 12发表: 2015年 ...
During viral infections, for example, apoptosis kills off invaded cells to hinder the spread of virus, sparing the whole organism. In neurobiology, this same phenomenon helps to prune synaptic connections, strengthening pathways that are used frequently, and disposing of the rarely used ones. As wi...
They also used STING Antibody (MAB7196) for WB analysis in experiments involving IL2/PHA-stimulated PBMCs, and this study established that activated T cells detect intracellular DNA through IFI16 and recruit STING as well as TBK1. Hansen et al. (2014) published a report in EMBO Journal, ...
Cells or tissues are crosslinked to preserve chromatin–protein interactions, and the protein of interest is immunoprecipitated with an antibody. Quantification of the precipitated DNA by PCR or profiling methods indicates enrichments of the protein of interest. Chromatin remodelers Enzymes or complexes ...
Bottom: single hPIEZO1 activity in HEK293T cells transfected with the same construct used for protein purification and representative histogram of single chan- nel events (liposome recording solution: 200 mM KCl, 40 mM MgCl2, 5 mM HEPES, pH 7.4. HEK293 cell recoding solution: 140 mM NaCl, ...
Class I is characterized by four ubiquitous and relatively small enzymes (∼500 amino acids) essentially located in the nucleus of cells [25, 26]. HDACs 1 to 3 are found in complexes with specific transcriptional co-repressors, blocking the expression of tumor suppressor genes [27]. Interest...
OAT2, encoded by SLC22A7, similarly is located in the baso- lateral membrane of the proximal tubule24), and its activity as a low-affinity urate transporter was found in transfected HEK293 cells25). Renal Hypouricemia Hypouricemia caused by a renal tubular defect wa...
Figure 1. On-target vs off-target effects of T-type calcium channel (TTCC) blockers used in cancer cells. Selective inhibition of TTCCs (indicated by white cross on inner mouth of channel) inhibits all Ca2+-dependent pathways in their signaling domain. Switched-off effectors/pathways are repr...