Two fetal cell lines that are being used in the development and production of several COVID-19 vaccines are HEK293 and PER.C6. HEK293 was derived from a fetus that was aborted in 1972. PER.C6 was isolated from an abortion that occurred in 1985. (No ongoing abortions need to be performe...
利用CRISPR/Cas9技术构建敲除MEIS2基因的HEK293T人胚肾细胞系 CRISPR/Cas9系统作为一种新型的基因组编辑技术,利用人工设计的向导RNA(singleguide RNA,sg RNA)介导外源表达的Cas9蛋白与基因组靶点特异性结合以实现对基因组DNA的特... 卢利莎,白杨,刘鑫,... - 《中国细胞生物学学报》 被引量: 12发表: 2015年 ...
aWe transfected HEK293T cells with Flag-ATXN1[82Q], fractionated cell extracts, and immunoprecipitated Flag-ATXN1[82Q] from each of the fractions using anti-Flag antibody (Supplementary Fig. S8b). 我们transfected HEK293T细胞与旗子ATXN1( 82Q),分馏了细胞萃取物,并且使用反旗子(抗体) ...
T-type Ca2+ channels Pharmacological blockers Repurposing Cancer cells Chemotherapy On/off-target effects Ca2+ signaling Apoptosis Autophagy Sorry, something went wrong. Please try again and make sure cookies are enabledReference 1. S.J. Roberts-Thomson, S.B. Chalmers, G.R. Monteith The calcium...
Acta Pharmacologica Sinica npg 94 www.nature.com/aps D'Onofrio PM et al While necrosis is more dominant in the ischemic core, apop- tosis becomes more common in the penumbra as the cells are found further away from the core[2]. Apoptosis is a normal process during development, and is ...
awe transfected myc-RBM17 with GST-ATXN1-S776, -S776A, or -S776D into HEK293T cells. 我们transfected myc-RBM17与GST-ATXN1-S776, - S776A或者- S776D入HEK293T细胞。 [translate] aMain bearing lubricating greases 主要轴承润滑脂 [translate] aExporter No 出口商没有 [translate] aIF ...
death stimuli. Over-expressing mutant forms of LRRK2 in primary neurons can lead to apoptotic cell death, but this is not the case for other cell lines we use to study LRRK2 function. HEK293T cells, for example, are resistant to mutant LRRK2 induced death,” says Rideout of his ...
Gedey and colleagues found that MVA activates ERK1/2 in HEK293T cells [191]. This is in contrast with the findings by Schweneker et al. [188] who showed that CVA induced sustained ERK 1/2 activation in HEK293 cells but MVA failed to do so. The authors demonstrated that ERK1/2 ...
Stromal cells are important factors for tumour cell expansion and the associated angiogenesis, which is modulated by the adenosinergic pathway. TGF-β has been shown to regulate the ADO-generating enzymes CD39, and CD73, which accelerated tumour progression by promoting the maturation of myeloid-deri...
Micropattemed glass slides were placed in a 10 cm cell culture petri dish and exposed to cell suspensions of HEK 293T and HepG2 at a concentration of 1x106 cells/ml. Micropatterning of ECM proteins on glass substrates to regulate cell attachment and proliferation On February 15, 2013 NASDA...