We analyzed 30 patients known to be positive with high/intermediate/low HCV RNA load, and kept their serum samples in storage at 4°C for 0, 1, 2, 3, 7, 14, 21 and 28 days. Quantitative detection of HCV RNA was performed by TaqMan real-time PCR. The results showed that no ...
For quantitative detection of HCV specific RNA using real-time RT-PCR Products Product Details Resources FAQ Products artusHCV RT-PCR Kits CE are intended for in-vitro diagnostic use. artusHCV RG RT-PCR Kit (96) Cat. No. / ID. / ID.4518265 ...
同时抗原消失的时间比RNA晚,所以当HCV RNA检测不到的时候,它也可以用来HCV感染的后期阶段的监测治疗。但是因为HCV cAg与HCV RNA相比,前者的敏感度较低,多项研究表明,该技术在低于5 000～10 000 IU/ml的HCV RNA下不存在反应性[36],在低病毒血...
【Abstract】ObjectiveTostudythecorrelationbetweenHCVRNAquantitationandanti-HCVlevelinthechronichepatitisCpatients.MethodsHCVRNAquantitationof95inthechronichepatitisCpatientsweredetectedbyauantitafedfluorescencequantitativereversetranscriptionpolymerasechainreaction(FQ-RT-PCR)andanti-HCVlevelweredetectedbychemiluminescenceimmuno...
INOGene-Hepatitis C Virus (HCV) Quantitative RT-PCR Kit is an in vitro nucleic acid amplification assay for quantification of Hepatitis C virus (HCV) RNA in human serum or plasma (EDTA) using viral RNA extraction kits via Real-Time PCR instrument. INOGene-Hepatitis C Virus (HCV) Quantitative...
2.1.1HCV RNA 检测方法采用的是实时定量PCR(Quantitative Real-time PCR)的方法;所用仪器为美国AB7500PCR实时荧光定量PCR仪;试剂厂商为中山大学达安基因股份有限公司。定量线性范围:5×102-108IU/mL;小于5×102IU/mL结果为阴性。大于5×102IU/mL结果为阳性,报具体定量数值结果。实验严格按照说明书进行,每天开展室内...
RT-PCR was performed for the 5′ non-cording region and was followed by nucleotide sequencing for HCV genotyping. Meanwhile, serum HCV-RNA concentration was detected by quantitative PCR. SPSS21.0 and Graphpad Prism 5.0 software were used for data analysis. Analysis of variance (ANOVA) was used ...
目前RQPCR检测HCV RNA在临床已广泛应用,RQPCR技术融合了PCR技术与DNA探针杂交技术的优点,实验的整个过程只在加样时打开1次反应管,在PCR的每个循环中可以直接监测到荧光信号的变化,根据PCR反应酶动力学特点,分析软件会自动对DNA进行定量,因此也有人称RQPCR为实时荧光定量PCR[6](realtime quantitative PCR)。其优点较多...
丙型肝炎病毒(HCV)是危害肝脏的主要病毒之一,造成严重的公共卫生问题。目前常用的HCV检测方法有血清学检测和分子生物学检测(HCV-RNA检测),血清学检测又可分为HCV核心抗原(HCV-cAg)检测和HCV抗体(HCV-Ab)检测。 HCV-Ab检测 HCV-Ab是机体由于HCV的刺激而产生的蛋白质,HCV-Ab检测...
丙型肝炎病毒核酸检测试剂盒(PCR-荧光法)COBAS® AmpliPrep/COBAS® TaqMan® HCV Quantitative Test, version 2.0,HCV磁性玻璃颗粒试剂盒、HCV裂解试剂盒、HCV 多元试剂盒、HCV 测试专用试剂盒、HCV 强阳性质控品、HCV 弱阳性质控品、COBAS TaqMan 阴性质控品(人血