不同HCV RNA定量检测法可用拷贝/ml和IU/ml两种表示方法,两者之间进行换算时,应采用不同检测方法的换算公式,如罗氏公司Cobas V2.0的IU/ml与美国国立遗传学研究所的SuperQuant拷贝数/ml换算公式是:IU/ml=0.854×拷贝数/ml+0.538。HCV载量的高低与疾病的严重程度和疾病的进展并无绝
400-821-3475 (服务热线) China - (English) Armored RNA Quant®Hepatitis C Virus (GT 2b) Control Product Guides and Listings Hepatitis C virus is a single-stranded RNA virus classified in the Flaviviridae family; HCV is classified into six genotypes (1-6) with several subtypes within each ...
Conclusions: The Xpert HCV Viral Load assay accurately quantifies HCV RNA regardless of the HCV genotype and can thus confidently be used to detect active HCV infection in serum and in whole blood specimens.doi:10.1016/j.jcv.2019.06.007Mélanie Wlassow...
Viral RNA was extracted from serum samples using the automatic Easy Mag extractor (Biomerieux, Lyon, France). Full-length HCV NS3/4 A sequences were obtained as previously described [14] while partial HCV NS5A and NS5B genes were amplified using the Transcriptor One-Step RT-PCR enzyme in ...
结论 双探针荧光定量提高HCV RNA检测的灵敏度和特异性,HCV RNA含量与丙型肝炎患者病情变化及严重程度相关。 【关键词】HCV RNA 双探针 荧光定量 丙型肝炎 Clinical significance of detection of HCV RNA by using double probed real-time fluorescence quantitiative determinatior. XU Bang-lao,WU Yu-xia,WANG...
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Briefly, 10 μl of extracted viral RNA was mixed with an internal control provided by Sacace HCV Real TM Quant kit and subjected to viral quantification using a real-time PCR SmartCycler II system (Cepheid Sunnyvale, USA). Total RNA isolation and gene expression analysis Total RNA from HCV ...
Performance evaluation of the Aptima HIV-1 RNA quant assay on the Panther system using the standard and dilution protocols J Clin Virol, 129 (2020), p. 104479 10.1016/j.jcv.2020.104479 View in ScopusGoogle Scholar 20. F Marcuccilli, S Chevaliez, T Muller, L Colagrossi, G Abbondanza, K ...
In May 2016, the World Health Organization adopted the first global hepatitis strategy, setting the ambitious goal of “elimination of viral hepatitis as a public health threat” by 2030. HCV-specific targets included a 65% reduction in HCV-related...
PCR (Polymerase chain reaction) – PCR tests measure HCV RNA in the blood. They can measure small amounts o virus [5-10 IU/mL]. bDNA (Branched-chain DNA) – The bDNA test only measures medium to high viral loads above 615 IU/mL. This ...