通过整合WGS、H3K27ac ChIP-seq、Hi-C等技术,分析结果发现许多基因由于SE与SV的重叠而表现出异常表达。这些异常表达在某些非CAGA LUAD病例中显著增加了术后复发的风险。总之,这篇文章识别到了大量的潜在的驱动基因,从而能更准确地选择临床试验患者,这有望提高LUAD的个性化治疗方法的有效性并改善患者预后。
A Sup. Figure S1 Hi-C VEGFA B VEGFA promoter VEGFA +157 enhancer Chr6 H3K4me1 H3K27Ac eRNA DHS Scale: Fig. S1: A. ChIP-seq tracks for H3K27ac and H3K4me1 including DHS at the VEGFA locus in K562 cells. VEGFA +157 enhancer is marked by the eRNA (GH06J043925) and is ...
染色质免疫沉淀 实验 (ChIP) - View 2 publications 肽阵列 (Array) 0.25 µg/mL - 产品规格 种属反应 Human 已发表种属 Human 宿主/亚型 Rabbit / IgG 分类 Polyclonal 类型 Antibody 抗原 Acetylated peptide (Lys27) corresponding to Human H3 (aa 24-31) 偶联物 Unconjugated 形式 Liquid...
We quantified ChIP material using Qubit, constructed ChIP–seq library with Nextera XT DNA Library Prep Kit (Illumina) according to the manufacturer’s instruction and sequenced for 101-bp paired-end reads by Illumina HiSeq 2500 at Broad Technology Labs (BTL) in the Broad Institute. BTL impleme...
ChIP-Seq data were generated on Illumina HiSeq X Sequencer. ChIP-Seq analysis The data quality control of ChIP-Seq data was performed same as RNA-seq. We used Bowtie2 (version 2.3.5.1) to gain read alignment by mapping reads against reference genome galGal6 (http://ftp.ensembl.org/pub...
The purified DNA were prepared for sequencing using the ChIP-Seq kit according to the manufacturer's protocol (Illumina, Inc., San Diego, CA). Sequencing was per- formed using the Illumine HiSeq2000 platform (Illumina, Inc., San Diego, CA). The raw ChIP-Seq data have been deposited into...
626 peaks were identified as LPS-increased – peaks for which the signal after LPS stimulation was higher compared to the control – in both ChIP-seq rep- licates (Group 1) while other groups of peaks showed less reproducible changes (Groups 2, 3 and 4) (Fig. 1b). No regions were ...
ChIP-Seq data were generated on Illumina HiSeq X Sequencer. Jiang et al. Biology of Sex Differences (2022) 13:6 Page 4 of 17 ChIP‑Seq analysis The data quality control of ChIP-Seq data was performed same as RNA-seq. We used Bowtie2 (version 2.3.5.1) to gain read alignment ...
Improved ChIP Sequencing for H3K27ac Profiling and Super-Enhancer Analysis Assisted by Fluorescence-Activated Sorting of Formalin-Fixed Paraffin-Embedded Tissues Nenggang Jiang Zhihao Wen Hongyan Liao Biological Procedures Online(2025) Nucleosome fibre topology guides transcription factor binding to enhancers...
[86], ChIA-PET, Hi-C (a method based on chromosome conformation capture), and Hi-ChIP (HiC chromatin immunoprecipitation) data in K562, MCF7 and GM12878 lymphoblastoid cells [87], OCEAN-C (Open Chromatin Enrichment And Network Hi-C) in U266 myeloma cells, RPMI-8226 multiple myeloma ...