调控基因/增强子生物学功能验证(ChIP-qPCR,双荧光素酶等) #总 结# H3K27ac作为增强子信号,在癌症和神经性疾病中有着重要影响。组蛋白修饰检测技术ChIP-seq、CUT&Tag和单细胞ChIP-seq可进一步为相关疾病的诊断和治疗提供新的靶点和策略。其中ChIP-seq、CUT&Tag可进行全基因组图谱绘制,而最新的单细胞ChIP-seq技术...
调控基因/增强子生物学功能验证(ChIP-qPCR,双荧光素酶等) 总结 H3K27ac作为增强子信号,在癌症和神经性疾病中有着重要影响。组蛋白修饰检测技术ChIP-seq、CUT&Tag和单细胞ChIP-seq可进一步为相关疾病的诊断和治疗提供新的靶点和策略。其中ChIP-seq、CUT&Tag可进行全基因组图谱绘制,而最新的单细胞ChIP-seq技术可更进...
Figure 2: SNAP-ChIP-qPCR data. Histone H3K27ac antibody (3 µg) was tested in a native ChIP experiment using chromatin from K562 cells (3 µg) with the SNAP-ChIP K-AcylStat Panel (EpiCypher 19-3001) spiked-in prior to micrococcal nuclease digestion. Specificity (left y-axis) was ...
EpiCypher是一家为表观遗传学和染色质生物学研究提供高质量试剂和工具的专业制造商。EpiCypher推出的ChIP级别的Histone H3K27ac Antibody符合EpiCypher的“SNAP-ChIP® Certified”标准,用于ChIP实验中的特异性和有效靶标富集(整板的交叉反...
Histone H3 acetyl Lys27 antibody tested by ChIP qPCR analysis. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) and HeLa Chromatin (1.5 x 10^6 cell equivalents per ChIP) using 10 ug of Histone H3K27ac pAb or rabbit IgG as a negative control. Real time, qua...
Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H3K27acChIP assays were performed using human HeLa cells, the Diagenode monoclonal antibody against H3K27ac (Cat. No. C15200184) and optimized PCR primer pairs for qPCR. ChIP was performed with the “Auto ...
使用针对H3K27ac的抗体(产品编号25239)和优化的qPCR引物组合,在HeLa细胞中进行ChIP实验。实验使用了100万细胞的剪切染色质。抗体用量梯度为1、2、5和10 ?g/次ChIP实验,并进行了分析。以2 ?g/IP的IgG作为阴性免疫沉淀对照。定量PCR使用了活性基因ACTB和EIF4A2的启动子引物作为阳性对照,以及非活性基因MYT1和TSH2B...
ChIP-qPCR of several genes confirmed that C/EBPβ binds to the target regions, recruits p300, and increases H3K27ac. Our genome-wide analysis revealed that C/EBPβ induces H3K27ac throughout the genome and upregulates gene expressions during decidualization by recruiting p300 to the promoters....
Detroit 562 cells were pre-treated with BAY 11–7082 or DMSO before stimulation with LPS for 80 min and ChIP-qPCR was performed for RELA. Results show the average percentage of input of two independent experiments, error bars are standard deviation. C: NFKBIA expression. Detroit 562 cells ...
The isolated gonads were either snapped frozen in liquid nitrogen and stored at -80℃ for RNA-Seq and RT-qPCR, or dissociated into cell suspensions for ChIP. At the same time, a small piece of embryonic heads or other tissues were collected and stored at −20 ℃ separately for PCR ...