Cell-type specific differences in the GTPγS binding assay described above led us to hypothesize the existence of a cell-specific factor that influenced the transfer of signal from R to G or the basal activation state of G independent of receptor. To pursue the latter, we modified the membran...
(2003) The [ 35 S]GTPgammaS binding assay: approaches and applications in pharmacology. Life Sci. 74 , 489–508.Harrison C, Traynor JR (2003) The [35S]GTPgammaS binding assay: approaches and applications in pharmacology. Life Sci 74(4):489–508...
Use of the GTPγS ([35S]GTPγS and Eu-GTPγS) binding assay for analysis of ligand potency and efficacy at G protein-coupled receptors (2010) Use of the GTPgammaS ([35S]GTPgammaS and Eu- GTPgammaS) binding assay for analysis of ligand potency and efficacy at G protein-coupled .....
G. (2010). "Use of the GTPγS ([35S]GTPγS and Eu-GTPγS) binding assay for analysis of ligand potency and efficacy at G protein-coupled receptors". British Journal of Pharmacology, 161(6), 1238-49. doi:10.1111/j.1476-5381.2010.00963....
L. (2004) Assay of G protein-coupled recep- tor activation of G proteins in native cell membranes using [35S]GTP␥S binding. Methods Mol. Biol. 237, 135-143Bidlack JM, Parkhill AL (2004) Assay of G protein- coupled receptor activation of G proteins in native cell membranes using [...
independent (constitutive) [[sup.35]S]GTP[gamma]S binding were sensitive to changes in assay concentrations of sodium, magnesium, and the guanine nucleotide GDP; however, the potency of ABT-239 for inhibition of RAMH-stimulated [[sup.35]S]GTP[gam...
The development of an assay for agonist-stimulated [ 35 S]guanylyl-5′-O-(γ-thio)-triphosphate ([ 35 S]GTPγS) binding, originally developed for receptors in isolated membranes, has provided an excellent opportunity to apply this process to brain sections, thus allowing the visualization of ...
In spite of this anomalous effect, l-SOP was found to be a competitive antagonist in the cAMP assay as well as in the [35S]GTP纬S binding assay with a pA2 value of 5.2 in both assays. MAP4 was a competitive antagonist of l(+)-2-amino-4-phosphonobutyric acid (l-AP4)-induced ...
The present study demonstrates that agonist stimulated [35S]GTPγS binding autoradiography could be a suitable technique for the examination of dopamine-D2/D3 receptors in the human brain. This functional assay could provide useful new information about dopamine receptor/Gprotein coupling in the ...
The primary ultra-high-throughput screen of the entire compound collection was accomplished on the Kalypsys (San Diego, CA) robotic platform at a concentration of 8 [micro]M using the 1,536-well [[sup.35]S]GTP[gamma]S SPA binding functional assay. The...