Cell-type specific differences in the GTPγS binding assay described above led us to hypothesize the existence of a cell-specific factor that influenced the transfer of signal from R to G or the basal activation
In order to set up a GTPgammaS binding assay, the requirements of the assay must be considered. These are tissue source, GTPgammaS analogue, G protein, GDP, Mg(2+) /Na(+) ions, saponin, incubation time. The assay, once optimized, can be used to generate concentration/response curves ...