two for nonspecific binding. The assay may also be done triplicate. Nonspecific binding is defined with 100μMGTPγS or Gpp(NH)p. The final concentration of GDP is 1–10μM. In the standard assay system, the preincubation mixture consists of brain G protein (125nM) and 60μg of membran...
In order to set up a GTPgammaS binding assay, the requirements of the assay must be considered. These are tissue source, GTPgammaS analogue, G protein, GDP, Mg(2+) /Na(+) ions, saponin, incubation time. The assay, once optimized, can be used to generat...
In order to set up a GTPgammaS binding assay, the requirements of the assay must be considered. These are tissue source, GTPgammaS analogue, G protein, GDP, Mg(2+) /Na(+) ions, saponin, incubation time. The assay, once optimized, can be used to generate concentration/response curves ...
Subtype-specific stimulation of [35S]GTPgammaS binding by recombinant alpha2-adrenoceptors. -(3-[]thiotriphosphate) ([]GTPγS) as a functional assay to monitor G-protein activation by recombinant human α-adrenoceptor subtypes α, α and α. (−... JM Peltonen,M Pihlavisto,M Scheinin -...
Hydrolysis of fluorescent GTP analogues BODIPY FL guanosine 5 '-O-(thiotriphosphate) (BGTPgammaS) and BODIPY FL GTP (BGTP) by Galpha(i1) and Galpha was characterized using on-line capillary electrophoresis (o) laser-induced fluorescence assays in order that changes in sub-strate, substrate-...
Cannabinoid ligands displayed differential abilities to maximally stimulate [35S]GTPgammaS binding in the presence of GDP. Efficacy differences among ligands increased with increasing GDP concentrations. GDP competition curves revealed that agonists induced low affinity GDP Ki values that were proportional ...
Hydrolysis of fluorescent GTP analogues BODIPY FL guanosine 5 '-O-(thiotriphosphate) (BGTPgammaS) and BODIPY FL GTP (BGTP) by Galpha(i1) and Galpha was characterized using on-line capillary electrophoresis (o) laser-induced fluorescence assays in order that changes in sub-strate, substrate-...
39, No.5, 583-593, October 2007 Inhibition of gamma ray-induced apoptosis by stimulatory heterotrimeric GTP binding protein involves Bcl-xL down-regulation in SH-SY5Y human neuroblastoma cells So-Young Kim1, Miran Seo1, Jung-Min Oh1, Eun-Ah Cho1 and Yong-Sung Juhnn1,2 1Department of ...
The role of Xgrip210 in gamma-tubulin ring complex assembly and centrosome recruitment. J. Cell Biol. 151, 1525–1536 (2000). 34. Demeret, C., Bocquet, S., Lemaitre, J. M., Francon, P. & Mechali, M. Expression of ISWI and its binding to chromatin during the cell cycle and ...
The present invention provides three novel GTP-binding proteins (designated individually as BND-1, BND-2, and BND-3, and collectively as BND) and polynucleotides which identify and encode BND. The invention also provides genetically engineered expression vectors and host cells comprising the nucleic...