Plasma membrane perforation elicited by caspase cleavage of the gasdermin D (GSDMD) N-terminal domain (GSDMD-NT) triggers pyroptosis. The mechanisms underlying GSDMD membrane translocation and pore formation are not fully understood. Here, using a proteomics approach, we identified fatty acid synthase ...
Fig : Western blot analysis of GSDMD N-terminal on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10007, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbi...
.; [Gasdermin-D, C-terminal]: Cytoplasm, cytosol . 组织表达: Expressed in the suprabasal cells of esophagus, as well as in the isthmus/neck, pit, and gland of the stomach, suggesting preferential expression in differentiating cells. GSDMD N-terminal rabbit pAb Catalog No PLA021446 Product...
为了进一步阐明候选药物对GSDMD介导的焦亡抑制作用的分子机制,作者在HEK293T细胞中进行了Darts实验,发现化合物68和98可以保护宽型GSDMD N端蛋白不被pronase降解,并增加其稳定性,而GSDMD N端寡聚化界面I处药物结合位点的突变则取消了化合物68和98对GSDMD N端蛋白稳定性的保护作用(图4A)。此外,作为阴性对照,寡聚化界...
2022年11月2日，南京医科大学杨硕课题组和南京中医药大学胡刚/王冰微课题组合作，在PNAS杂志发表题为“The Gasdermin D N-terminal fragment acts as a negative feedback system to inhibit inflammasome-mediated activation of Caspase-1/11”的研究论文，揭示了固有免疫细胞焦亡效应分子GSDMD负反馈调控炎症小体激活的...
2022年11月2日,南京医科大学杨硕课题组和南京中医药大学胡刚/王冰微课题组合作,在PNAS杂志发表题为“The Gasdermin D N-terminal fragment acts as a negative feedback system to inhibit inflammasome-mediated activation of Caspase-1/11”的研究论文,揭示了固有免疫细胞焦亡效应分子GSDMD负反馈调控炎症小体激活的作...
2022年11月2日,南京医科大学杨硕课题组和南京中医药大学胡刚/王冰微课题组合作,在PNAS杂志发表题为“The Gasdermin D N-terminal fragment acts as a negative feedback system to inhibit inflammasome-mediated activation of Caspase-1/11”的研究论文,揭示了固有免疫细胞焦亡效应分子GSDMD负反馈调控炎症小体激活的作...
2022年11月 2日,南京医科大学杨硕课题组和南京中医药大学胡刚/王冰微课题组合作,在PNAS杂志发表题为The Gasdermin D N-terminal fragment acts as a negative feedback system to inhibit inflammasome-mediated activation of Caspase-1/...
2022年11月2日,南京医科大学杨硕课题组和南京中医药大学胡刚/王冰微课题组合作,在PNAS杂志发表题为“The Gasdermin D N-terminal fragment acts as a negative feedback system to inhibit inflammasome-mediated activation of Caspase-1/11”的研究论文,揭示了固有免疫细胞焦亡效应分子GSDMD负反馈调控炎症小体激活的作...
2022年11月2日,南京医科大学杨硕课题组和南京中医药大学胡刚/王冰微课题组合作,在PNAS杂志发表题为“The Gasdermin D N-terminal fragment acts as a negative feedback system to inhibit inflammasome-mediated activation of Caspase-1/11”的研究论文,揭示了固有免疫细胞焦亡效应分子GSDMD负反馈调控炎症小体激活的作...