Center line, mean; box bounds, upper and lower quartile; whisker, maximal and minimal value (n = 5). c Levels of full-length or the truncated N-terminal GSDMD protein in control and Mll4−/− B16 bulk tumors are shown as mean ± SEM (sgVector, n = 10; Mll4KO, ...
Purified GSDMA3 containing a engineered PPase cleavage site between the gasdermin N and C domain was cleaved in vitro to obtain GSDMA3(N + C). a, Coomassie blue staining of the prepared GSDMA3 proteins. b, ATP-based viability of CT26 cells electroporated with the prepared GSDMA3 protein. ...
Cell lysates containing recombinant FLAG-hGSDMD were incubated with human neutrophil lysate for the times indicated. The positions of the full-length (FL-GSDMD) and ELANE-cleaved N-terminal hGSDMD (hGSDMD-NT) are indicated. (D) Cleavage of mouse GSDMD (mGSDMD) by mouse neutrophil lysate. ...
After synthesis, LC3 protein is cleaved by Atg4 to expose a glycine residue, forming the cytosolic LC3-I. During autophagy, LC3-I undergoes ubiquitin-like conjugation and processing to be coupled with PE to form LC3-II, which then localizes on the inner and outer membranes of autophagosomes...
Therefore, we believed that the strategies of actively delivering intact GSDM family proteins or their N-terminal to induce tumor cell pyroptosis were more feasible. One way to achieve this is through the delivery of GSDM-N to tumor cells using extracellular vesicles-based GSDMD-N mRNA delivery...
(PGPSt) improved the structure of mouse hepatocytes; attenuated hepatocellular injury; significantly reduced AST, ALT, and SOD activities and the levels of IL-6, IL-1β, TNF-α, and MDA in liver tissues; downregulated the expression of cleaved caspase-3 and Bax in liver tissues; upregulated...
Pyroptosis-related proteins (NLRP3, GSDMD, cleaved caspase-1 and IL-1β) were markedly highly expressed in the liver cirrhosis model ((G,J), *** p < 0.001 vs. the control). The disulfram inhibited the expression of pyroptosis-related proteins, as shown in (H). The expression levels ...
decreased compared to the CCl4group ((M), ****p< 0.0001). NLRP3, GSDMD, cleaved-caspase-1 and IL-1β expression in the Exos group was significantly decreased compared to that in the CCl4group ((N–R), *p< 0.05). The data are displayed as the means ± SD; n = 5 for each ...