Movie S2. 293T Cells Expressing Caspase-Cleaved NT Fragment (mGSDMD-1 to -275), Related to Figure 7 Movie S3. 293T Cells Expressing ELANE-Cleaved NT Fragment (mGSDMD-1 to -268), Related to Figure 7 Movie S4. 293T Cells Expressing an Inactive Form of NT Fragment (mGSDMD-1 to -225...
a, b, Preparation of the GSDMA3(N + C) used for conjugation onto the nanoparticle. Purified GSDMA3 containing a engineered PPase cleavage site between the gasdermin N and C domain was cleaved in vitro to obtain GSDMA3(N + C). a, Coomassie blue staining of the prepared GSDMA3 proteins....
6f, g). In addition, Decitabine treatment impairs tumor progression in immunocompetent mice and gives rise to a marked increase of both full-length and the cleaved N-terminal fragment of GSDMD in bulk melanomas in vivo (Supplementary Fig. 6h–k), further substantiating a key role of DNMT3A...