融合蛋白的街头序列GS-linker的碱基序列是什么怎么查找啊？比如GGGGS的碱基序列，真核表达体系 ...
Agarose gel electrophoresis of the amplified DNA fragments of VH, VL, Cκ, GS Linker and assembled scFv library, assembled ribosome display library.Haiwei, ChengYifei, ChenYi, YangXueqiu, ChenXiaolu, GuoAifang, Du
A SAGE experiment is performed by digesting cDNA with the frequent cutting restriction enzyme NlaIII, isolating the most 3' fragment and ligating a linker containing the sequence TCCGAC, which is recognized the restriction enzyme MmeI. Tags are generated by MmeI which cleaves the DNA strand 20/1...
We also characterized DPGs among other eukaryotic genomes [see Additional file2]. The majority of these DPGs have TSS distance s from0 to 400 bp amongDmeland vertebrates [see Additional file3]. In addition, vertebrates have a relatively higher proportion of DPGs with overlapping sequences. The...
(28)An exact numerical method to calculate the base-unpairing probability for any given DNA sequence by Benham model,Thin Solid Film,2006,通讯作者 (29)Predicting the function of eukaryotic scaffold/matrix attachment regions via DNA mechanics,J. Phys.: Condens. Matter,2005,通讯作者 ...
试验例2:b、p、o序列顺序将b、p、o通过连接肽串联在一起,顺序可以互换,但是如果b与o相连,则linker数目为4,p与o相连,则linker数目为1,如果b与p相连,则linker数目为4,分别命名为b4p1o、b4o1p、o4b4p、o1p4b、p1o4b和p4b4o,按照实施例1的方法,制备m2-3e-gs,其中,b4p1o蛋白的结果如图4所示。按照试验例...
cbcrg/tcoffee - A collection of tools for Multiple Alignments of DNA, RNA, Protein Sequence TimoLassmann/kalign - A fast multiple sequence alignment program. refresh-bio/FAMSA - Algorithm for ultra-scale multiple sequence alignments (3M protein sequences in 5 minutes and 24 GB of RAM) Darkabo...
(19)Targeting of parallel c-myc G-quadruplex by dimeric cyanine dye supramolecular assembly: dependence on the linker length,Analyst,2015,第11作者(20)Multicomponent self-assembled metal-organic 3 rotaxanes,J. Am. Chem. Soc.,2015,第11作者(21)Directly lighting up RNA G-quadruplexes from test ...
For creating the HSA/PTH (1-34) fusion protein [Genbank accession no.JN711437], the C-terminus of HSA and the N-terminus of PTH (1-34) were genetically linked by a flexible linker GlyGlyGlyGlySer, as previously reported [4]. In this study, to secrete the fusion protein with its na...
The deduced amino acid sequences showed that the ExgS has a non-homologous linker region consisting of 180 amino acids, which encompassed highly conserved regions observed in Exg homologues from filamentous fungi. A recombinant protein (ExgS) has been recovered from the cultural filtrate of an ...