融合蛋白的街头序列GS-linker的碱基序列是什么怎么查找啊？比如GGGGS的碱基序列，真核表达体系 ...
Agarose gel electrophoresis of the amplified DNA fragments of VH, VL, Cκ, GS Linker and assembled scFv library, assembled ribosome display library.Haiwei, ChengYifei, ChenYi, YangXueqiu, ChenXiaolu, GuoAifang, Du
After the plasmid was generated, the DNA sequence of the insert was verified. For the dual-luciferase reporter gene assay, ND7/23 cells were cultured in 10-cm cell culture dishes to 70–80% density. The cells were co-transfected with 10.5 μg of the expression vectors for both an ARE-...
10, panels C and D), a residue located in the C-terminal end of Switch I region near the junction with linker 2 (19, 20). Cys200 of Gsα is embedded in a sequence highly conserved among G protein α subunits Leu Arg Cys Arg Val Xaa Thr, including Giα1, Giα2, and Goα. ...
cbcrg/tcoffee - A collection of tools for Multiple Alignments of DNA, RNA, Protein Sequence TimoLassmann/kalign - A fast multiple sequence alignment program. refresh-bio/FAMSA - Algorithm for ultra-scale multiple sequence alignments (3M protein sequences in 5 minutes and 24 GB of RAM) Darkabo...
(1)Kinesin Neck Linker is Optimally Designed to Achieve the largest stepping velocity: a Simulation Study; (2)A Molecular Simulation Study on the Allosteric Mechanism of Na+ - coupled symporter BetP. 8, “关于步行马达kinesin结构优化的一点思考”,邀请报告,海峡两岸生物学启发的理论科学问题研讨会(BIT...
(11)Structure and sequence analysis-based engineering of pullulanase from Anoxybacillus sp. LM18-11 for improved thermostability,J Biotechnol,2015,第4作者 (12)Studies on properties of the xylan binding domain and linker sequence of xylanase XynG1 1 from Paenibacillus campinasensis G1 1,J Ind Micr...
Nucleotide sequence and properties of the murine γ3 immunoglobulin heavy chain gene switch region: implications for successive Cγ gene switching During B lymphocyte differentiation, inmmoglobulin heavy chain constant region (CH) genes undergo a unique series of DNA recombination events cu... Lawrence...
The deduced amino acid sequences showed that the ExgS has a non-homologous linker region consisting of 180 amino acids, which encompassed highly conserved regions observed in Exg homologues from filamentous fungi. A recombinant protein (ExgS) has been recovered from the cultural filtrate of an ...
(45)Discovery of potent 2,4-difluoro-linker poly(ADP-ribose) polymerase 1 inhibitors with enhanced water solubility and in vivo anticancer efficacy.,Acta Pharmacol Sin.,2017,第6作者 (46)Potent, Selective, and Cell Active Protein Arginine Methyltransferase 5 (PRMT5) Inhibitor Developed by Structure...