However, when we made a single nucleotide change in each (GGGGS) linker we were able to achieve expression The change of one nucleotide within each linker did not alter either the amino acid sequence or the frequency score of these codon triplets' usage in mammalian cells. Instead they ...
内容提示: Molecular Immunology 40 (2004) 717–722Optimization of codon pair use within the (GGGGS) 3 linkersequence results in enhanced protein expressionRyan Trinh, Brian Gurbaxani, Sherie L. Morrison ∗ , Manouchehr SeyfzadehDepartment of Microbiology, Immunology and Molecular Genetics and the ...
(GGGGS) in Pichia pastoris. Keywords linker;human serum albumin;Pichia pastoris 人血清 白蛋 白(human serum albumin,HSA)是人 血浆中含量最高的蛋 白质,具有无免疫原性 、人体相容 性好 、组织分 布广 、无 酶活 等特性 ,在血 浆 中的半 衰期 可达 14～20 d,使其成为非常理想 的药物融 合载体...