This observation demonstrates that fusions with glycine-serine linker sequences should be carefully evaluated during drug development to prevent the introduction of a phosphorylation site in therapeutic fusion proteins.KEYWORDS: Alkaline phosphatase, CID, EThcD, fusion protein, Glycine-serine ...
Typically, a flexible interdomain linker sequence connects the catalytic domain(s) to the CBD (Korndorfer et al., 2006). Nearly all Gram-positive phage endolysins and autolysins are the products of single genes, although group I introns are often found within these genes and have been ...
Another high frequency mutation site was positioned in the linker sequence, A108G in glyOFF5 and A108U in glyOFF12 (Additional file 1: Table S9), which might affect the cooperative mode of the two aptam- ers. These eight novel mutations (A56G, U79C, C143A, U177C, U179A ...
Nevertheless, we also find that RBD-1 specificity is not completely normal because it bound a SELEX sequence that lacks U-runs more strongly than full-length Sxl (Fig. 5 C, compare 15 and S5A). The discrepancy may arise from differences in extent of RBD-1 where we excluded the linker ...
Amino acid sequence and identification of the N epsilon-lipoyllysine residue. J Biol Chem 261:8836–8841 Gonzalez IL, Tugendreich S, Hieter P, Sylvester JE (1993) Fixation times of retroposons in the ribosomal DNA spacer of human and other primates. Genomics 18:29–36 Hiraga K, Kochi H,...
(GmMYB176–GmbZIP5) using an 18 bp linker DNA (5′-AGCACAACATTTCAACCA-3′)25, and expressed them in soybean hairy roots. Overexpression ofGmMYB176, GmbZIP4, andGmbZIP5in GmMYB176–GmbZIP4 and GmMYB176–GmbZIP5 hairy roots were verified by comparing their expression levels with ...
The first method involves the use of linkers, which are short pieces of double-stranded DNA of known nucleotide sequence. The linker, which contains a restriction site, can be ligated to the DNA molecules by using an excess quantity of the linker in the ligation reaction. Digestion of the re...
The linker sequence may, e.g., comprise a sequence which promotes better folding of the target protein and/or a cleavage site for cleaving off the purification tag. A“helix structure” is characterized by having an amino acid sequence which results in a coiled structure stabilized by ...
Fluorescent protein tagged hepatitis B virus capsid protein with long glycine-serine linker that supports nucleocapsid formationChenJiang-YanGanChun-yangCaiXue-feiZhangWen-luLongQuan-xinJOURNAL OF VIROLOGICAL METHODS
Detection of a phosphorylated glycine-serine linker in an IgG-based fusion protein.doi:10.1080/19420862.2016.1236165Oksana TyshchukHans Rainer VlgerClaudia FerraraPatrick BulauHans KollMichael MlhjTaylor & Francis