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Samples of fish fillet (25 g) were homogenized for 120 s with a laboratory blender (Stomacher 400, Seward, London, England) in a stomacher bag containing 225 mL of sterile peptone water (0.1%, w/v, peptone and 0.9%, w/v, NaCl). The homogenate was centrifuged at 700 rpm for 2 min...