引人注目的是,MBOAT2过表达,而不是PLA2G2F,能够不含Trolox而维持HT1080-GPX4KO细胞的长期培养,这让人想起亲脂性自由基诱捕抗氧化剂(RTA)生成酶FSP 1、GCH 1和NOS 2的GPX4非依赖性抗铁死亡功能(图1 I和S1B-S1D)。我们观察到FSPl在HT 1...
为了进一步验证2-BP和ML349通过靶向GPX4来调节铁蛋白沉积的敏感性,团队生成了GPX4基因敲除的A375细胞,然后将这些细胞皮下注射到裸鼠体内进行异种移植试验。用IKE单独或与2-BP或ML349联合治疗A375 GPX4-KO异种移植肿瘤,为期4周。与之前的研究一致,GPX4-KO...
MBOAT1 是一种额外的 MUFA 首选铁死亡抑制 LPLAT,受雌激素受体 (ER) 调节 在HT1080-GPX4KO 细胞中,即使 MBOAT2 被敲除,SCD1 过表达也可以促进细胞存活,这表明存在也可以催化 PL-MUFA 合成的其他 LPLAT。 有 14 个已鉴定的 LPLAT,其中 10 个来自 1-acylglycerol-3-phosphate O-acyltransferase (AGPAT) 家...
日前,山东大学贾继辉教授研究团队在《Cell Death & Differentiation》(2021 IF:15.828)在线发表了题为“Wnt/beta-catenin signaling confers ferroptosis resistance by targeting GPX4 in gastric cancer”的研究结果。该研究探究了在幽门螺杆菌感染的过程中Wnt/β-catenin信号通路与铁死亡之间的关系,阐明幽门螺杆菌激活Wnt/...
为了证明TCF4通过GPX4调控胃癌细胞铁死亡,研究者构建了GPX4基因敲除的胃癌细胞系,并分别在对照细胞与GPX4-KO细胞中过表达TCF4,检测脂质过氧化产物MDA的水平与胃癌细胞的集落形成能力。结果显示,过表达TCF4能够降低胃癌细胞脂质过氧化水平,增强胃癌细胞集落形成的能力;而在GPX4被敲除的情况下,过表达TCF4对胃癌细胞铁死亡的...
Cancer Cell 26, 455–464 (2014). Article CAS PubMed PubMed Central Google Scholar Koepp, D. M. et al. Phosphorylation-dependent ubiquitination of cyclin E by the SCFFbw7 ubiquitin ligase. Science 294, 173–177 (2001). Article CAS PubMed Google Scholar Yada, M. et al. Phosphorylation...
Davis, R. J., Welcker, M. & Clurman, B. E. Tumor suppression by the Fbw7 ubiquitin ligase: mechanisms and opportunities.Cancer Cell26, 455–464 (2014). CASPubMedPubMed CentralGoogle Scholar Koepp, D. M. et al. Phosphorylation-dependent ubiquitination of cyclin E by the SCFFbw7 ubiquitin...
Featured Product KD/KO Validated CoraLite® Plus 488-conjugated GPX4 Monoclonal antibody GPX4 Monoclonal Antibody for IF/ICC Cat No. CL488-67763 CloneNo. 3F5G5 Publications(1) Host / Isotype Mouse / IgG2b Reactivity Human, mouse, rat, rabbit, pig Applications IF/ICC and More (1) ...
GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form ...
为了证明TCF4通过GPX4调控胃癌细胞铁死亡,研究者构建了GPX4基因敲除的胃癌细胞系,并分别在对照细胞与GPX4-KO细胞中过表达TCF4,检测脂质过氧化产物MDA的水平与胃癌细胞的集落形成能力。结果显示,过表达TCF4能够降低胃癌细胞脂质过氧化水平,增强胃癌...