Binding of an agonist to the seven-transmembrane G-protein-coupled receptor in the plasma membrane activates a pathway that involves G proteins as well as cAMP-related pathways that modulate cellular signaling. In this example, the activated G alpha (Gαi/0) proteins inhibit (-) adenylyl cyclas...
2024年2月2日,北大-清华生命科学联合中心自振滔和饶毅发表一篇论文,主要三点:首先,GPR39有新的配体:胆酸;其次,发现胆酸作用的新信号途径;第三,发现胆酸可以通过GPR39而引起急性胰腺炎。 GPR39是七重跨膜的G蛋白偶联受体(GPCRs)之一。...
15). We hypothesize that the observed conformations of the holoreceptor can activate a certain signaling pathway while the same conformations are inactive for the other signaling pathways and vice versa. This potential signaling bias opens new directions for further studies on aGPCRs. An ECR-...
noxious heat-induced intracellular Ca2+release from ER and action potentials of DRG neurons largely reduced when ablating TMC6 or blocking Gαq/PLC/IP3R signaling pathway as well. In summary, our findings indicate that TMC6 can directly function as a Gαq-coupled GPCR-like receptor...
et al. NPC1-mTORC1 signaling couples cholesterol sensing to organelle homeostasis and is a targetable pathway in Niemann-Pick type C. Dev. Cell 56, 260–276.e7 (2021). Article CAS PubMed Google Scholar Shin, H. R. et al. Lysosomal GPCR-like protein LYCHOS signals cholesterol sufficiency...
(B) Snake diagram of APH1A. The serine residues that display increased (red) or decreased (blue) levels of phosphorylation in each GRK KO cell line are indicated. (C) βarr2 recruitment to APH1A in the CHO-βarr2 cell line following expression of APH1AWT or the APH1A ICL2 and C-te...
Whether the G protein is in the GTP- or GDP-bound state and for how long determines the extent of signal that will be transmitted through the pathway (5) and therefore requires tight regulation. Crucial regulatory molecules influencing the nucleotide-bound state of Gα subunits are the ...
When there is one (two, or three) nucleotide difference, there is one (two, or six) possible pathway between the codons. In these cases, the number of synonymous and nonsynonymous differences for the j-th codon, s d,j and n d,j respectively, can be calculated by considering all ...
In PANC-1 cells, although GPCRs stimulate Golgi PI4P hydrolysis and PI4P contributes the majority of the total IP produced, an alternative pathway is involved in PKD activation. Overall we conclude that PI4P hydrolysis at the Golgi is a major reaction stimulated by GPCRs. This mechanism ...
FIG. 11 is a block diagram of a multiprocessor computer system using a shared virtual address memory space. FIGS. 12A-12C depict sequences of immunoglobulin scaffolds. FIG. 13 depicts sequences of G protein-coupled receptors scaffolds.