Purification of a sample of the labeled peptide and the application of MS/MS to sequence the peptide usually allows the identification of a unique labeled carboxyl residue as the enzyme nucleophile; a result which may be confirmed through Edman degradation. The catalytic nucleophiles of over 2...
machinery for glycan synthesis and degradation as these underlie dysregulated glycosylation. AI is particularly powerful in many fields, but it has been more challenging to deploy in glycobiology for a few important reasons. However, opportunities are emerging that can start to address these (Table 1...
Ermonval M et al. (2001) N-glycan structure of a short-lived variant of ribophorin I expressed in the Madia214 glycosylation-defective cell line reveals the role of a mannosidase that is not ER mannosidase I in the process of glycoprotein degrada...
Ermonval M et al. (2001) N-glycan structure of a short-lived variant of ribophorin I expressed in the Madia214 glycosylation-defective cell line reveals the role of a mannosidase that is not ER mannosidase I in the process of glycoprotein degrad...
Phase separation (or liquid-liquid phase separation, LLPS) refers to the phenomenon that biological macromolecules (such as proteins, RNA, etc.) form a temporary liquid-liquid phase separation state in the cell.39Phase separation is a widespread liquid state in physical chemistry. In cells, phase...
Phase separation (or liquid-liquid phase separation, LLPS) refers to the phenomenon that biological macromolecules (such as proteins, RNA, etc.) form a temporary liquid-liquid phase separation state in the cell.39Phase separation is a widespread liquid state in physical chemistry. In cells, phase...
Here, we report that the glycosylation-deficient mutant of PAT1 ( PAT13 NQ) is unstable and is degraded mainly via the endoplasmic reticulum-associated degradation pathway in HEK293 cells. Interestingly, PAT13 NQ binds preferentially to the plasma membrane rather than to the lysosome. Consistent ...
Heterosides (glycosides) are biological compounds that yield upon hydrolysis one or more sugars and a nonsugar component, the aglycone. Chemically speaking, the glycosides are acetals in which one hydroxyl group of the sugar moiety is condensed with a hydroxyl group of a nonsugar component. Both...
Glycosylation sites identified by solid-phase Edman degradation: O-linked glycosylation motifs on human glycophorin A The human red blood cell sialoglycoprotein, glycophorin A (GpA), contains a 'mucin-like' extensively O-glycosylated extracellular domain which carries the ... P Anthony,JW Redmond,KL...
Ermonval M et al. (2001) N-glycan structure of a short-lived variant of ribophorin I expressed in the Madia214 glycosylation-defective cell line reveals the role of a mannosidase that is not ER mannosidase I in the process of glycoprotein degrad...