PREPARATION OF WORKING SOLUTION Add 5 mL of Assay Buffer (Component C) into a bottle of Enzyme Mix (Component B) and mix well. Add 25 µL of Amplite™ HRP substrate stock solution into the bottle of Component B + C and mix them well to make glycerol working solution (Component A ...
The oil red O stock solution was equilibrated for more than 3 days, and the 60% diluted solution was equilibrated overnight and filtered two times on the day of the experiment. Images were taken using a Leica microscope and were analyzed using image J. Immunoblotting For the phospho-AMPK ...
Mortimer RK, Johnston JR: Genealogy of principal strains of the yeast genetic stock center. Genetics 1986, 113: 35-43. CAS Google Scholar Thomas BJ, Rothstein R: Elevated recombination rates in transcriptionally active DNA. Cell 1989, 56: 619-630. 10.1016/0092-8674(89)90584-9 Article CAS ...
coli refers to genetically unmodified MG1655 (ATCC 47076) stock strain. Two single knock-in strains (GLPK, glpK 218a4t; RPOC, rpoC 27 bp del), as well as a double knock-in strain (DKI, glpK 218a4t þ rpoC 27 bp del; a reconstruction strain of a glycerol- evolved clone) were ...
Nostoc sp. strain Yaku-1 from a stock culture (10% glycerol, −80 °C) was streak-cultured on the plate at 23 °C for 2–4 weeks under 16-h photoperiodic conditions (white LED, 2,000 lux). At 2- to 3-week intervals, this purification process was repeated until only ...
coli refers to genetically-unmodified MG1655 (ATCC 47076) stock strain. The GlpK mutant strains were derived in a previous study by introducing individual glpK mutations into the wild-type genome by λred recombination (5, 17). Purification of Mutant Glycerol Kinase Enzymes (GlpK) Cloning into ...
A stock suspension of each liposome containing 4.8×1014liposomes per ml was diluted to give an injection suspension containing 12×106liposomes per ml. The liposomal suspensions were used to inject into mice to determine the effect on ear swelling in the murine CHS model, a biological system use...
RNA Extraction, Preparation of cDNA Library, and Sequencing Total RNA was extracted using the mirVana miRNA Isolation Kit (Ambion) following the manufacturer’s protocol. RNA integrity was evaluated with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Samples with RNA Inte...
Pastor-Pérez and Sepúlveda-Escribano [52] used Sn as the promoter in the preparation of the Pt-based catalysts; the influence of Sn addition on the activity, H2 selectivity, and stability in the glycerol steam reforming was investigated. As suggested by XPS and TPR-H2 analyses, the ...
A stock solution of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA; 160 µL, Gelest, Inc., Morrisville, PA, USA) was added to the dried samples followed by incubation for 20 min. Heptane (1.6 mL) was then added to the samples, and they were transferred to autosampler vials for ...