Genetic engineering bacteria producing glycerol dehydrogenase (GDH) and GDH preparation methodThe invention belongs to the field of biotechnology, which comprises the following steps: adopting a PCR technology t
The respiratory pathway for glycerol utilization in bacteria begins with the phosphorylation of glycerol into G3P by the ATP-dependent GK, which is followed by the dehydrogenation of G3P into DHAP by two respiratory GPDHs, GlpABC and GlpD [90,91]. The former GPDH, encoded by the glpABC ...
Fresh media cultures are inoculated by dipping into the frozen stock with a sterile loop or pipette. Assay ≥99.5% (GC), corrected for water Concentration 0.8 Suitability Suitable for cell culture, electrophoresis, and insect cell culture Keywords Aliphatic Compounds, Aloe Vera, Carbohydrates...
Take 60 ± 1 mg sample into the 2 mL EP tubes, extracted with 0.48 mL extraction liquid (VMethanol: VH2O = 3: 1), add 20 μL of adonitol (0.5 mg mL−1 stock in dH2O) as internal standard, vortex mixing for 30 s; Homogenized in ball mill for 4...
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High SA titers and productivities have been achieved in industrial fermentations using bacteria [5]. However, a drawback of using bacteria for organic acid production is their low tolerance towards acidity [10]. In contrast, fungi (including yeasts) tolerate low pH values, reducing the need for...
Bacteria were isolated from soil contaminated with used cooking oil in Thailand. The bacterial strains were maintained by monthly subculturing on nutrient agar slants. Stock cultures were maintained at −80 °C in a 15 % (v/v) glycerol solution. Carbon sources Crude glycerol and refined glycero...
The stock solution (1000 μg/mL) for was prepared for each compound and screened for antimicrobial activity by diluting the stock solution from 1000, 500, 250, 125, 62.5, 31.25, 15.62 up to 7.8 μg/mL. The tubes along with the control were then kept for incubation at 37 °C for 24...
Biological processes for the preparation of glycerol using carbohydrates or sugars are known in yeasts and in some bacteria, other fungi, and algae. Both bacteria and yeasts produce glycerol by converting glucose or other carbohydrates through the fructose-1,6-bisphosphate pathway in glycolysis. In ...
The invention relates to the development of appropriate cultivation conditions for a bacteria to grow anaerobically (fermentatively) on a glycerol substrate. The method requires cul