Genetic engineering bacteria producing glycerol dehydrogenase (GDH) and GDH preparation methodThe invention belongs to the field of biotechnology, which comprises the following steps: adopting a PCR technology to clone a gldA gene from klebsiella pneumoniae DSM 1115, constructing an Escherichia coli ...
Fresh media cultures are inoculated by dipping into the frozen stock with a sterile loop or pipette. Assay ≥99.5% (GC), corrected for water Concentration 0.8 Suitability Suitable for cell culture, electrophoresis, and insect cell culture Keywords Aliphatic Compounds, Aloe Vera, Carbohydrates...
The respiratory pathway for glycerol utilization in bacteria begins with the phosphorylation of glycerol into G3P by the ATP-dependent GK, which is followed by the dehydrogenation of G3P into DHAP by two respiratory GPDHs, GlpABC and GlpD [90,91]. The former GPDH, encoded by the glpABC ...
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Effect of whole human versus bovine milk and formula on growth of bacteria We tested the human milk, bovine milk samples, and the commercial infant formula sample for ability to inhibit the growth of selected Gram-positive aerobesS. aureus(a potential gastrointestinal pathogen known for its roles...
Bacteria were isolated from soil contaminated with used cooking oil in Thailand. The bacterial strains were maintained by monthly subculturing on nutrient agar slants. Stock cultures were maintained at −80 °C in a 15 % (v/v) glycerol solution. Carbon sources Crude glycerol and refined glycero...
Take 60 ± 1 mg sample into the 2 mL EP tubes, extracted with 0.48 mL extraction liquid (VMethanol: VH2O = 3: 1), add 20 μL of adonitol (0.5 mg mL−1 stock in dH2O) as internal standard, vortex mixing for 30 s; Homogenized in ball mill for 4...
The glycerol-producing medium was a sterilized medium (pH 6.7) of 8 g of KH2PO4, 2 g of Na2HP4, 0.75 g of (NH4)2SO4, 8 g of (NH4)2HPO4, 6.6 g of citric acid 2.05 g of MgSO4, 40 mg of CaCl2, 40 mg of FeSO4, and a trace element stock solution, in 1 L distilled wat...
Palsson7 & Martin Robert1,2,w Comparative whole-genome sequencing enables the identification of specific mutations during adaptation of bacteria to new environments and allelic replacement can establish their causality. However, the mechanisms of action are hard to decipher and little has been achieved...
After adding 1.5% gellan gum powder (Wako, Osaka, Japan), the solution was autoclaved at 121 °C for 15 min, at which point the mixture was shaken well, and cast in plastic petri dishes. Nostoc sp. strain Yaku-1 from a stock culture (10% glycerol, −80 °C) was streak-...