Bacterialglycerolstocks To 2mls of mid-log culture or 1ml of freshly saturated culture add 1 ml(or an equal volume) of glycerol solution, mix gently, then freeze rapidly in liquid nitrogen or on dry ice. Store at -20°C and - 70°C. Glycerol solution: (Per 100 ml) 65% glycerol (v...
other nasty critter. If the plates are old, either transform a new set of cells or chip of a bit of frozen glycerol stock from the top of the tube with a pipet tip (do NOT let the frozen cell thaw) and spread on an LB Agar plate with antibiotic. Culture overnight in the 37 ...
Prior to the incubation, the bacterial isolates were cultivated from glycerol stocks as described before. In the case of challenged isolates, 20 μM of either IV or MX - or 10 μM in the case of S. pneumoniae (02-MXc) and S. mitis (01-MXc) - was added to the culture medium,...
S. typhimuriumSL7207 strains expressing scrambled-shRNA and cMyc-shRNA were revived from glycerol stocks 12 h before scheduled co-culture. A scraping of a glycerol stock was placed in 5 ml of LB broth with kanamycin (50 μg/ml) and grown in a shaking incubator at 37 °C for 8 h. 3...
S. typhimuriumSL7207 strains expressing scrambled-shRNA and cMyc-shRNA were revived from glycerol stocks 12 h before scheduled co-culture. A scraping of a glycerol stock was placed in 5 ml of LB broth with kanamycin (50 μg/ml) and grown in a shaking incubator at 37 °C for 8 h. ...
Two days prior to the experiment, 10 µL per well of sterile LB broth was added to each well and a multichannel pipette was used to inoculate this semi-solid master plate from the arrayed glycerol stocks. For bacteria panned after growth on solid media, a flame-sterilized loop was ...
To test whether fusA mutations could be present at very low frequency in planktonic populations, glycerol stocks of evolved planktonic populations were grown overnight and concentrated 10 times before being plated on 2x amikacin MIC plates. Among the clones growing on these plates, we could indeed ...
The following day colonies were grown in LB and stored as glycerol stocks. The L. monocytogenes EGDe chiB gene (lmo0105) and a truncated version of chiB (lmo1883ΔCBM) lacking the C-terminal carbohydrate binding domain (amino acids 711–755) were amplified through PCR with primers designed ...
Strains were routinely grown at 37 °C, 220 rpm, in 5 ml of L-broth (LB) medium (1% NaCl, 1% tryptone, 0.5% yeast extract, 1.5% agar for solid media) and long-term stored at −80 °C in 20% glycerol stocks. Sequencing was performed by Eurofins Genomics (Ebers...
Frozen storage of equine FMT material can preserve potentially metabolically active bacteria of the equine fecal microbiome, with saline plus glycerol preservation more effective than saline alone. Larger studies are needed to determine if these findings apply to other individual horses. The ability to ...