Bacterialglycerolstocks To 2mls of mid-log culture or 1ml of freshly saturated culture add 1 ml(or an equal volume) of glycerol solution, mix gently, then freeze rapidly in liquid nitrogen or on dry ice. Store at -20°C and - 70°C. Glycerol solution: (Per 100 ml) 65% glycerol (v...
S. typhimuriumSL7207 strains expressing scrambled-shRNA and cMyc-shRNA were revived from glycerol stocks 12 h before scheduled co-culture. A scraping of a glycerol stock was placed in 5 ml of LB broth with kanamycin (50 μg/ml) and grown in a shaking incubator at 37 °C for 8 h. 3...
S. typhimuriumSL7207 strains expressing scrambled-shRNA and cMyc-shRNA were revived from glycerol stocks 12 h before scheduled co-culture. A scraping of a glycerol stock was placed in 5 ml of LB broth with kanamycin (50 μg/ml) and grown in a shaking incubator at 37 °C for 8 h. 3...
glycerol stocks of evolved planktonic populations were grown overnight and concentrated 10 times before being plated on 2x amikacin MIC plates. Among the clones growing on these plates, we could
Two days prior to the experiment, 10 µL per well of sterile LB broth was added to each well and a multichannel pipette was used to inoculate this semi-solid master plate from the arrayed glycerol stocks. For bacteria panned after growth on solid media, a flame-sterilized loop was ...
This work demonstrates and guides how to use a range of state-of-the-art artificial neural-networks to analyse bacterial microscopy images using the recently developed ZeroCostDL4Mic platform. We generated a database of image datasets used to train netwo
Proteins were arrayed at a concentration of ∼40 µm in a buffer that contains 20% glycerol (v/v) to prevent evaporation. A small amount (typically 100 nm) of cyanine-5-labeled bovine serum albumin (BSA) was combined with each protein to facilitate image analysis. After a 12-h ...
分析测试,百科网,StreakingBacterialStocks, rinciple:Bacterial cells are streaked onto a medium to obtain an independent isolate. This is done to reduce the likelihood of working with a culture which has become contaminated
IACUC protocol #PROTO202101789. All V. fischeri strains used in this study were derived from either ES114 or FQ-A001, and regularly maintained on LBS medium (defined below) at 28°C.44,45 Glycerol stocks of V. fischeri strains were maintained at -80°C. Strains were regularly authenticated...
Septins are cytoskeletal proteins that assemble into hetero-oligomeric complexes and sense micron-scale membrane curvature. During infection with Shigella flexneri, an invasive enteropathogen, septins restrict actin tail formation by entrapping bacteria