A small RNA, i.e., tRNAGly, was regarded to be able to enhance transcription of the sgRNA as a potential enhancer of Pol III and also ensure precise release of sgRNA spacer-scaffold structure from the sgRNA expression cassette [27, 28]. In addition to change of the promoter, in recent...
theA. niger5S rRNA along with its 338-bp upstream promoter sequence, an HDV ribozyme, and a sgRNA scaffold were all amplified from the plasmid psgRNA4.0 as described earlier [25]. The second construct was constructed by replacing the HDV ribozyme with the tRNAGlyfragment (sequence listed in A...
Class I enzymes approach tRNA from the acceptor stem minor groove side, while class II synthetases bind the major groove side of the tRNA acceptor stem. Because of the way class I enzymes interact with their tRNAs, an unwinding of the acceptor stem is needed for the tRNA 3′-end to reach...