The kit is suitable for Glutathione S-transferase activity determination in cell lysates, tissues, etc. Principle The Glutathione S-transferase Assay Kit is based on the GST enzyme reaction between GSH and the GST substrate, CDNB (1- chloro-2,4-dinitrobenzene). The GST catalyzed formation of...
23,24. While mechanisms of resistance to Venetoclax that directly affect BCL-2 have been explored, changes influencing the cellular pharmacokinetics of BCL-2 inhibitors and their accumulation in leukemic target cells are largely unknown.
In summary, our findings uncover a previously unknown link between the ER resident and UPR sensor protein IRE1α and ferroptosis regulation independent of UPR activation and IRE1α’s canonical downstream effector XBP1. Previous studies reported that determination of cellular sensitivity to ferroptosis i...
An aliquot of the supernatant was used for total protein determination with the bicinchoninic acid assay kit (Sigma–Aldrich, St. Quentin Fallavier, France). The remaining supernatant was deproteinated by mixing with an equal volume of metaphosphoric acid reagent (Sigma– Aldrich) and centri...
2.2. Determination of protein content We used the Bradford protein assay to determine the protein content of isolated mitochondria, with bovine serum albumin as the standard. Enzyme activities are expressed per milligram of protein. 2.3. Isolation of yeast mitochondria Yeast cells were cultured overnig...
Anderson, “determination of Glutathione and Glutathione Disulfide in Biological Samples”, Methods in Enzymology, vol. 113, pp. 548-555, 1985. International Search Report issued with respect to application No. PCT/JP2015/055276, dated May 19, 2015. International Preliminary Report on Patentabilit...
Determination of Glutathionylation Activity—To monitor the glutathionylation activity of Grx, deglutathionylated substrate peptide was added to GSSG in the presence and absence of enzyme. Upon addition of a glutathione moiety, a decrease of 69 arbitrary units in total fluorescence was observed with...
The present invention provides an assay for detection of oxidized glutathione (GSSG) and the determination of the ratio of GSH and GSSG in a cell. BACKGROUND OF THE INVENTION To remain healthy, cells, in particular mammalian cells, need to maintain a balance between oxidizing and reducing condit...
Determination of the target value is based on predictive automatic gain control. Survey scans were acquired at a resolution of 70,000 at m z−1 200, and the resolution for HCD spectra was set to 17,500 at m z−1 200. The instrument was run with peptide recognition mode enabled....
Determination of GSR activity The enzyme activity of GSR was analyzed using a GSR assay kit (S0055, Beyotime). Prepared cells were washed twice and lysed with a cell lysate. Extracted GSR catalyzes the reduction of GSSG to GSH upon the consumption of NADPH in the reaction system. GSH react...