1 KIT CN¥3,543.13MAK453-1KT CN¥3,543.13 Availability Estimated to ship onJune 27, 2024Details Add to CartRequest a Bulk OrderRecommended Products Slide 1 of 10 Sigma-Aldrich CS0410 Glutathione-S-Transferase (GST) Assay Kit View Price and Availability Sigma-Aldrich MAK085 Lipid Peroxidatio...
Protein interactions in astrocytes were detected using the Duolink® PLA assay kit (Sigma-Aldrich, DUO92101) following the manufacturer’s protocol. After treatments, cells on slides were fixed with 4% paraformaldehyde and permeabilized with 0.3% Triton X-100 in PBS. Blocking solution was added ...
QIAGEN 9019325 Kit, heater FETw/drivers, RG6QIAGEN 9019234 Gasket - Cartridge, PyroQ24QIAGEN 9234760 Manual, user, hardware BR3000 intQIAGEN 9234735 Manual, user, hardware BR3000 USQIAGEN 9234703 Mikrowin data reduction software, upg.QIAGEN 9233721 Rack for Biovue Cards (holds 6 cards)QIAGEN ...
Confirmatory GPX1 enzyme-linked immunosorbent assay (ELISA) Kit analysis was then performed, and the results demonstrated that GPX1 expression in the PT group was significantly decreased compared to the sham group, whereas allicin significantly increased the expression level of GPX1 in the infarct ...
Briefly, bacterial cells were extracted with 1000 μL of cold methanol, which contained 10 μL of 0.1 mg/mL ribitol (Sigma) as internal analytical standard. The cells were lysed by sonication for 10 min at 30% intensity and were centrifuged for 10 min at 12,000 g at 4 °C. Then, ...
Glutathione, cysteine, ninhydrin, methionine, sorbitol, and GSSG were obtained from Sigma. Amplex red kit for glutamate estimation was obtained from Roche Applied Science. Restriction enzymes, Vent DNA polymerase, and other DNA modifying enzymes were obtained from New England BioLabs. PAGE protein ...
QIAGEN 1054575 HiSpeed Plasmid Giga EF Kit (5) QIAGEN 1061072 AIV-H5 RT-PCR REAGENT QIAGEN 1061094 HCV RT-PCR REAGENT VII QIAGEN 1061115 PG FMDV RT-PCR Assay (48) Z QIAGEN 9016711 Security Key, LC Analyzer, encoded QIAGEN 9016685 Washer Mechanical, RCS1 ...
20-mM RC DC stock in water and stored frozen in protein assay kit (Bio-Rad, cat. no. aliquots at –20 1C 500-0122) to estimate proteins . . . without interference from detergent or reducing agent LB broth containing 100 mg ml–1 ampicillin (Sigma) 6Â SDS loading buffer Buffer X:...
and 600 μl isopropanol, before frozen at –80 °C for at least one hour. After thawing, the DNA pellet was washed with 1 ml cold 70% ethanol, air-dried, and suspended in 600 μl ultrapure H2O overnight. The gDNA was quantified using Qubit dsDNA HS Assay Kit (Thermo Fischer)...
Synthesis of cDNA was carried out with the GeneAmp RNA PCR kit. In short, RNA (1 μg) was retrotranscribed for 15 min at 42 °C with 2.5 units of murine leukemia virus reverse transcriptase, using random hexamers. The enzyme was inactivated by heating for 5 min at 99 °C. Each RNA ...