Enzymatic Assay of GLUTATHIONE-S-TRANSFERASE (EC 2.5.1.18) p-Nitrobenzyl Chloride as SubstrateTest Blank
Rapid and accurate HPLC assay for plasma total homocysteine and cysteine in a clinical laboratory setting. Clin. Chem. 45, 290–292 (1999). CAS PubMed Google Scholar Pastore, A. et al. Fully automated assay for total homocysteine, cysteine cysteinylglycine, glutathione, cysteamine, and 2-merca...
(NOS), glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD), thioredoxin reductase (TrxR), catalase (CAT)). Assays for activity assessment of these enzymes are now available on the market and are widely used in research. Besides, certain enzymes can be used as...
3d). Activation of GAPDH required GSH, providing evidence that strep-MBP-ROXY9 can function as a weak GSH-dependent reductase rather than just as an acceptor of glutathione in this assay. ROXY9 shows a lower reactivity towards GSSG as compared to GRXC2 To elucidate whether the lack of ...
Enzymatic assay for glutathione These procedures have been tested extensively on kidney and are probably applicable to tissues in general.doi:10.1016/0003-2697(76)90323-7Jacqueline E. ... JE Brehe,HB Burch - 《Analytical Biochemistry》 被引量: 617发表: 1976年 Enzymatic solubilization of fish pro...
Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method. The spectrophotometric/microplate reader assay method for glutathione (GSH) involves oxidation of GSH by the sulfhydryl reagent 5,5'-dithio-bis(2-nitrobenz... I Rahman,A Kode,SK Biswas...
the aid of glutathione reductase produces GSH, which turns on the fluorescence of TPE-DCV, the ensemble of TPE-DCV and GSSG can thus serve as a label-free sensor for enzymatic activity assay of glutathione reductase. We also apply TPE-DCV for the detection of intracellular GSH in living ...
At 24-h intervals, 100 μl of the assay solution was mixed with 4.7 ml of 75% aqueous ethanol, 0.1 ml of ammonium thiocyanate (30% w/v) and 0.1 ml of 0.02 M ferrous chloride dissolved in 1 M HCl. An aliquot (200 μl) of this solution mixture for each sample was added to ...
ELISA enzyme-linked immunosorbent assay TrxR1 Thioredoxin Reductase 1 Sec Selenocysteine GSH Glutathione DTNB 5,5′-dithiobis-(2-nitrobenzoic acid) Tm Melting temperature BCA Bicinchoninic acid DSF Differential scanning fluorimetry SecTRAPs Selenium compromised thioredoxin reductase-derived apoptotic proteins ...
Cell debris was removed by centrifugation at 18,000 × g for 10 minutes at 4 °C. Protein concentration was measured with a BCA assay using supernatant. Enzymatic digestion One milligram of proteins from tissues, cells, or standard glycoproteins, including bovine serum fetuin (Millipore ...