In a 3.00 ml reaction mix, the final concentrations are 75 mM potassium phosphate,2.6 mM ethylenediaminetetraacetic acid, 1 mM glutathione, 0.09 mM ß-nicotinamide adeninedinucleotide phosphate, reduced form, 0.13% (w/v) bovine serum albumin, and 0.03 - 0.06 unit of glutathione reductase....
A sensitive fluorimetric microassay for the determination of glutathione peroxidase activity. Application to human blood platelets. Anal Biochem. 1979;98(1):154–9. Article CAS PubMed Google Scholar Mohammed A, Gutta V, Ansari MS, Saladi Venkata R, Jamil K. Altered antioxidant enzyme activity ...
Semiquantitative bioluminescent assay of glutathione. A novel technique has been developed for semiquantitative detection of glutathione (GSH) in small volumes of liquid samples. GSH is detected via enzymatic ... FJ Romero,W Mueller-Klieser - 《Journal of Bioluminescence & Chemiluminescence》 被引量...
the aid of glutathione reductase produces GSH, which turns on the fluorescence of TPE-DCV, the ensemble of TPE-DCV and GSSG can thus serve as a label-free sensor for enzymatic activity assay of glutathione reductase. We also apply TPE-DCV for the detection of intracellular GSH in living ...
This study attempts to investigate the relationship between skin sensitization assessed in the local lymph node assay (LLNA) initially and a thiol reactivity index based on glutathione (GSH), pEC(50) thiol (EC(50) being defined as the concentration of the test substance which gives 50% ...
[16]. O2* - radical is responsible for lipid peroxidation and to decrease the activity of antioxidant defense system enzymes such as catalase (CAT) and glutathione peroxidasee (GPx). It also causes damage to the ribonucleotide which is required for DNA synthesis. The protonated form of O2*- ...
As the cleavage of GSSG with the aid of glutathione reductase produces GSH, which turns on the fluorescence of TPE-DCV, the ensemble of TPE-DCV and GSSG can thus serve as a label-free sensor for enzymatic activity assay of glutathione reductase. We also apply TPE-DCV for the detection of...
Accumulation of reduced NADP in the assay was verified by spectral analysis and by finding rapid disappearance of absorbance at 340 nm on addition of glutathione reductase and oxidized glutathione into the reaction mixture. The method necessitates prior removal of glucose from the samples. This we ...
Refolding was monitored and optimized by determining the hydrolysis of the chromogenic substrate S-2288 as described later. The unfolded/purified FSAP was added dropwise to 100 mM Tris (pH 7.5), 125 mM MgCl2, 100 mM KCl, 2 mM reduced Glutathione (GSH), 1 mM oxidized Glutathione ...
mixture was vigorously vortexed for 20 seconds, followed by incubation on ice for 15 minutes. This cycle was repeated three times. Cell debris was removed by centrifugation at 18,000 × gfor 10 minutes at 4 °C. Protein concentration was measured with a BCA assay using ...