Measurement of oxi- dized glutathione by enzymatic recycling coupled to bioluminescent detection. Anal Biochem 2000; 283: 146-152.Tamara Mourad,Kyung-Lyum Min,Jean-Paul Steghens.Measurement of Oxidized Glutathione by Enzymatic Recycling Coupled to Bioluminescent Detection[J]. Analytical Biochemistry ....
A new method is described for the quantification of oxidized glutathione (GSSG) in tissues by enzymatic recycling coupled to NADPH bioluminescent detection. Tissue samples are treated with metaphosphoric acid. In a first step, after derivatization of GSH with 4-chloro-7-trifluoromethyl-1-methylquino...
Kadokawa Y, Ohba K, Omagari K, Akazawa S, Hayashida K, Ohnita K, Takeshima F, Mizuta Y, Kohno S: Intracellular balance of oxidative stress and cytoprotective molecules in damaged interlobular bile ducts in autoimmune hepatitis and primary biliary cirrhosis: In situ detection of 8-hydroxydeoxy...
建议收到Glutathione, Reduced/Oxidized (GSH/GSSG) Detection BioAssay Kit产品后将其置于4°C/-20°C保存。其他 Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体...
The most sensitive RPLC/FL method was presented by Guo et al. (2009) by using 1,3,5,7-tetramethyl-8-phenyl-(4-iodoacetamido)difluoroboradiaza-s-indacene (TMPAB-I). A limit of detection as low as 0.3 nm was achieved for GSH using an isocratic method and a precolumn ...
Assay of gamma-glutamyl-cysteine synthetase and glutathione synthetase in erythrocytes by high-performance liquid chromatography with fluorimetric detection.J Chromatogr 1990;530:122–8. CAS PubMed Google Scholar Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH. Measurement of protein using ...
Since enzymatic reduction is not possible due to the reaction of OPA with NAD(P)H and other stronger reducing agents, leading to an overestimation of GSSG levels, dithionite was used to reduce GSSG. High sample throughput combined with sensitive (20-pmol limit of detection) and accurate ...
Detection PrincipleGlutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be...
The advent of whole genome sequencing and high throughput screens enabled the detection of synthetic lethal interactions involving GSH metabolism inhibition. Indeed, whereas most cancer cells are resistant to GSH depletion, cells with specific genetic abnormalities are particularly vulnerable. The discover...
Select all/Deselect all Export citations Select all Front Matter Full text access Front Matter Copyright List of Contributors Preface Acknowledgment Properties and Organic Chemistry The Methods for Detection and Assay of Glutathione and Sulfhydryl Compounds ...