Measurement of Oxidized Glutathione by Enzymatic Recycling Coupled to Bioluminescent Detection[J] . Tamara Mourad,Kyung-Lyum Min,Jean-Paul Steghens.Analytical Biochemistry . 2000 (2)MOURAD, T., MIN, K.-L. e STEGHENS, J.-P.‚ 2000. Measurement of oxidized glutathione by enzymatic recycling ...
A new method is described for the quantification of oxidized glutathione (GSSG) in tissues by enzymatic recycling coupled to NADPH bioluminescent detection. Tissue samples are treated with metaphosphoric acid. In a first step, after derivatization of GSH with 4-chloro-7-trifluoromethyl-1-methylquino...
86. [IF=4.105] Cong Wang et al."Heteronuclear MOF-based fluorescent sensor for the detection of tetracycline antibiotics."APPLIED ORGANOMETALLIC CHEMISTRY L-谷胱甘肽 - 性质 可信数据 白色粉末或结晶性粉末。能溶于水、稀乙醇、氨水和二甲基甲酰胺,不溶于乙醇、乙醚和氯仿、丙酮。熔点195℃。 广泛分布于...
For each experimental run, 5 μL of extraction solvent were run in triplicate as blank controls; these were disbursed throughout the run to signal changes in the integrity of metabolite detection. The LC-MS metabolite peaks were manually identified and integrated by El-Maven (Version 0.10.0)...
Kadokawa Y, Ohba K, Omagari K, Akazawa S, Hayashida K, Ohnita K, Takeshima F, Mizuta Y, Kohno S: Intracellular balance of oxidative stress and cytoprotective molecules in damaged interlobular bile ducts in autoimmune hepatitis and primary biliary cirrhosis: In situ detection of 8-hydroxydeoxy...
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Determination of cellular thiols and glutathione-related enzyme activities: versatility of high-performance liquid chromatography–spectrofluorimetric detection. J. Chromatogr. B 719, 37–46 (1998). Article CAS Google Scholar Jocelyn, P. The standard redox potential of cysteine–cystine from the thiol...
The selection of reductant depends on the separation and detection system used. Sulphydryl-containing reducing agents, such as dithioerythritol, dithiothreitol and mercaptoethanol, liberate glutathione from various disulphides. These reductants form adducts with thiol-specific reagents and therefore may ...
Briefly, each primary antibody was incubated for 12 h in a humidified chamber at 37 °C, followed by detection using the HRP-conjugated secondary antibody and TSA-dendron-fluorophores (Histova, NEON 7-color IHC Kit for cryosection, 1:100), after which the primary and secondary antibodies...
Plant hormones and antioxidant system changes occur during plants' exposure to stress conditions. Although the interactions of some plant hormones (abscisic acid, salicylic acid, jasmonic acid, nitric oxide, and ethylene) with the glutathiones-transferase (GST) enzyme, which is one of the antioxidant...