The selection of reductant depends on the separation and detection system used. Sulphydryl-containing reducing agents, such as dithioerythritol, dithiothreitol and mercaptoethanol, liberate glutathione from various disulphides. These reductants form adducts with thiol-specific reagents and therefore may ...
high performance liquid chromatography with spectrophotometric detectionThe antithyroid properties of sulfur-containing amino acids cysteine and methionine and glutathione tripeptide are studied by evaluating the kinetic and thermodynamic parameters of analyte interaction anionic iodine complexes ( ({ ext{I}}_{...
Detection was by UV at 210 nm in HPLC, and by iodine vapors in TLC. The solvent conditions from TLC were transferred to open column chromatographic separation. Quantitative determination was carried out using TLC and column chromatography supplemented with UV spectrophotometry. Recovery was in the ...
A smartphone-integrated dual-mode nanosensor based on Fe<inf>3</inf>O<inf>4</inf>@Au for rapid and highly selective detection of glutathione 2022, Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy Citation Excerpt : It is clinically used in the treatment, and adjuvant thera...
The nonspecific protein detection was reduced by blocking membranes in TBS solution containing 25 mM Tris base, 150 mM NaCl, 5% nonfat milk, and 0.1% Tween 20. The membranes were then incubated with polyclonal antibodies against IR-β and IRS-1 and their phosphorylated forms in the ...
After protein removal, reduction of GSSG and inhibition of self-oxidation, various detection procedures were described using spectrophotometry, fluorimetry, high-performance liquid chromatography (HPLC), mass spectrometry or electrochemical analysis [12]. To the best of our knowledge, there is no ...
An indirect determination of GSH by the current of oxygen reduction in the Na2SO3medium in the concentration range from 0.5 × 10–8to 4.2 × 10–8mol/dmm3with a detection limit of 2.5 × 10–9mol/dm3is proposed. The developed voltammetric method is approved for the determination of GSH ...
A facile, sensitive, and selective colorimetric detection method for the disinfectant, didecyldimethy-lammonium chloride (DDAC) in aqueous samples was developed using glutathione (GSH)– functionalized gold nanoparticles (GSH–AuNPs). DDAC shorten the interparticle distance and immediately aggregate the ...
However, this reaction is significantly suppressed by cysteine and glutathione. Thus, a sensitive colorimetric method is presented based upon this inhibition. Excellent performance with low detection limits, good selectivity, and rapid analysis suggests potential bioanalytical applications for the determination...
Thus, when encapsulated in a silica matrix, PCn can be good candidates for heavy metal detection. (C) 2016 Elsevier Ireland Ltd. All rights reserved.Chemico-biological interactionsJacquart, AurelieBrayner, RobertaChahine, Jean-Michel El HageNguyet-Thanh Ha-Duong...