Therefore, the determination of the GSH/GSSG ratio and the quantification of GSSG are useful indicators of oxidative stress in cells and tissues.Principle of the Procedure:The low concentration of GSSG (high GSH/GSSG ratio) in tissues coupled with the need to prevent GSH oxidation during sample ...
The kit is suitable for Glutathione S-transferase activity determination in cell lysates, tissues, etc. Principle The Glutathione S-transferase Assay Kit is based on the GST enzyme reaction between GSH and the GST substrate, CDNB (1- chloro-2,4-dinitrobenzene). The GST catalyzed formation of...
The homogenates were centrifuged at 4 °C for 20 min at 12,000 g, and the supernatants were used for the determination of enzymatic activity. SOD activity was measured in a reaction mixture containing 50 mM phosphate buffer (pH 7.8), 0.1 mM EDTA, 13 mM methionine, 75 ...
A colorimetric micro-method for the determination of glutathione. Biochem J 1965;94:705–11.10.1042/bj0940705Search in Google Scholar 8. Guntherberg H, Rost J. The true oxidized glutathione content of red blood cells obtained by new enzymic and paper chromatographic methods. Anal Biochem 1966;15...
Determination of gastric secretion The effect of DES on gastric acid output was determined following the recommended method [21]. Briefly, Sprague Dawley rats assigned equally into five groups (n = 6). After 24 h fasting, immediately after pylorus ligature, 5% Tween 80, omeprazole (30 mg/kg)...
(pH 7.8) containing 0.2 mM EDTA, 2 mM AsA, and 2% PVP. The homogenates were centrifuged at 4 °C for 20 min at 12,000 g, and the supernatants were used for the determination of enzymatic activity. SOD activity was measured in a reaction mixture containing 50 mM ...
Substrate specificity determination showed differences the interaction of the enzyme with several hydrophobic substrates [81]. Hepatic SeGST substrate specificity pattern is not analogous. The apparent low substrate specificity to cumene hydroperoxide and hydrogen peroxide indicates it has inefficient peroxidase...
2.5. Total protein determination Total protein in all samples was quantified by mixing each sample with diluted (5x) Coomassie dye (Bio-Rad) in 96-well plates, with subsequent measurement of absorbance at 595 nm in a microplate reader [28]. Protein concentration was calculated from a calibration...
Determination of Catalytic Effects of aMTD/SD-Fused GPX7 Recombinant Proteins on GIT-Cancer Cell Apoptosis Apoptotic cells were analyzed using TUN EL assay with In Situ Cell Death Detection kit TMR red (Roche, 4056 Basel, Switzerland). Human gastric cancer cells (AGS and MKN45) and human oesop...
According to this principle, H2O2 content of pakchoi leaves was measured using the H2O2 determination Kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) following the manufacturer's instructions. 2.6. RNA Isolation, cDNA Library Preparation, Transcriptome Sequencing, RNA Sequencing Data ...