普洛麦格 ( 北京 ) 生物技术有限公司 Glucose Uptake-Glo 中 文 说 明 书 适用产品目录号：J1341、J1342和J1343 2020 版 CTM467原英文技术手册TM467Glucose Uptake-Glo™ Assay G9711, G9712 and G9713
Inhibition of glucose uptake results in a reliance on Complex I function for survival All substrates that drive TCA flux and NADH regeneration converge on Complex I (CI) of the ETC. To investigate KL-11743-induced changes in CI activity, we used an in-gel activity assay to evaluate endogenous...
The non-radioactive 2-dexoyglucose uptake was detected using a Glucose Uptake-GloTM Assay Kit (J1341, Promega, Madison, USA) according to manufactured instruction. Briefly, cells were seed on 96-well plates and serum starved for 3 h before the assay. Cells were washed with PBS three tim...
Luminescence from accumulated 2DG 6-phosphate was detected by use of the Glucose Uptake-Glo Assay (Promega Corporation, Madison, WI, USA). 2.8. Assays for NADPH To evaluate the involvement of PPPs within cells after the treatment of SGLT2 inhibitors, the content of NADPH in MT-2 cells was...
A glucose uptake assay was performed in KGN cells and hLGCs following serum starvation for 12 h and insulin stimulation (Sigma-Aldrich, 100 nM, 20 min) using the Glucose Uptake-Glo assay kit J1342 (Promega, Madison, WI, USA) according to the manufacturer’s instructions. RIP assay The RIP...
(glo͞o′kōs′) n. 1.A monosaccharide sugar, C6H12O6, that is used by living things to obtain energy through the process of aerobic respiration within cells. It is the principal circulating sugar in the blood of humans and other mammals. ...
Caspase 3/7 activity assay. Caspase 3/7 activity was measured using caspase-Glo 3/7 assay kit (Promega). Equal number of live cells from different treatment conditions was used for the assay. Cells were incubated with substrates for 1 h at room temperature and a luminescent signal was ...
Assay Buffer and transferred to 96-well assay plate at 1 × 104cells/well. Finally, fluorescence was quantified on Glomax Multi+Microplate Multimode Reader (Promega, Madison, WI) as previously described29,30. Monitoring glucose uptake in C2C12 and HepG2 cells where the expression of DNA...
Thereafter, cells were processed for MTT assay, Colony formation assay, Glucose utilization assay, Indirect ELISA, Immunofluorescence or Cell cycle analysis or for lysate preparation for western blotting, Glucose uptake assay, Dual luciferase assay and RNA extraction, as mentioned below in individual ...
Two days later the Dual-Glo Luciferase Assay System (E2940, Promega) was used to measure the luciferase activity of the cells on a bio-luminometer, following the manufacturer´s protocol. To avoid interference from firefly luminescence, all the samples on the 96-well plate were measured ...