An In Vitro Method to Analyze Glucose Uptake in PodocytesPodocytes are terminally differentiated, insulin-sensitive cells of the glomerular filtration barrier, with a central role in filtration barrier maintenance. Podocyte injury is one of the earliest......
In vitro proliferation evaluation with an MTT assay and a live-cell imaging system To evaluate the proliferation of the two cell lines under various in vitro conditions, we followed the common MTT evaluation assay33. The MTT assay is based on the conversion of MTT into formazan crystals by liv...
In human normal liver cell line L-O2 in vitro, treatment with BRB or BRBG (5, 20, 50 μmol/L) increased glucose consumption, enhanced glycogenesis, stimulated the uptake of the glucose analog 2-NBDG, and modulated the mRNA levels of glucose-6-phosphatase and hexokinase. However, both ...
Uptake assays by using radio isotope method, such as glucose uptake; GTPγS binding assays for GPCR agonists screen; Radioimmunoassay experiment, metabolite identification experiment and other customized detection requirements. Radioligand binding assay of A2aR (GPCR) Radioisotope assay for epigenetics targe...
Diseases caused by trypanosomatids include leishmaniasis (Leishmania spp.), Chagas disease (Trypanosoma cruzi), and sleeping sickness (Trypanosoma brucei) that affect millions of people, especially low-income populations, being classified as neglected tr
brasiliensis, larvae 3 stage (L3)- and larvae 5 stage worm (L5)-derived proteins, could induce an alternative activated macrophage phenotype with anti-inflammation properties in vitro [25]. Based on previous studies, we hypothesized ...
Our findings were contrary to these results and may be due to differences between in vivo and in vitro experiments, as well as experimental conditions. Our results suggest a possible mechanism by which increased arsenate uptake in STZ-induced diabetic mice was associated with PIT1 and PIT2 ...
in vitro assays with response profiles that imitate top features of dissociated cell populations from principal tumours thus indicating these represent disease-relevant useful assays for the testing of immune system checkpoint inhibitors in current and upcoming advancement. Such in vitro assays could also...
condition (two male and two female).eSchematic of glucose uptake assay, illustrated using Biorender software under license.fRepresentative images and (g) line scan analysis of PKD cysts after perfusion with fluorescent NBD glucose or unlabeled PBS control (mean ± s.e.m.,n ≥ 17 ...
Glutamine uptake/consumption was calculated by deducting the measured glutamine concentration in the medium from the original glutamine concentration. All values were normalized according to cell number. The in vivo tumorigenesis assay Five- to six-week-old BALB/c athymic nude mice (nu/nu, female) ...