普洛麦格 ( 北京 ) 生物技术有限公司 Glucose Uptake-Glo 中 文 说 明 书 适用产品目录号：J1341、J1342和J1343 2020 版 CTM467原英文技术手册TM467Glucose Uptake-Glo™ Assay G9711, G9712 and G9713
Glucose uptake The non-radioactive 2-dexoyglucose uptake was detected using a Glucose Uptake-GloTM Assay Kit (J1341, Promega, Madison, USA) according to manufactured instruction. Briefly, cells were seed on 96-well plates and serum starved for 3 h before the assay. Cells were washed with...
Most recently, Kadmon Corporation developed a pan Class I GLUT1-4 inhibitor (KL-11743) through a cell-based phenotypic assay assessing inhibition of non-mitochondrial ATP production followed by structure–activity relationship optimization [26]. While KL-11743 effectively blocks glucose uptake in the ...
In 3T3-L1 adipocytes, DNAJB3 has a role in improving the sensitivity to insulin and glucose uptake. In co-immunoprecipitation assays, DNAJB3 interacts with both JNK1 and IKKβ kinases. However, the functional impact of such interaction on their activities has not been investigated. Here, we ...
A glucose uptake assay was performed in KGN cells and hLGCs following serum starvation for 12 h and insulin stimulation (Sigma-Aldrich, 100 nM, 20 min) using the Glucose Uptake-Glo assay kit J1342 (Promega, Madison, WI, USA) according to the manufacturer’s instructions. RIP assay The RIP...
Luminescence from accumulated 2DG 6-phosphate was detected by use of the Glucose Uptake-Glo Assay (Promega Corporation, Madison, WI, USA). 2.8. Assays for NADPH To evaluate the involvement of PPPs within cells after the treatment of SGLT2 inhibitors, the content of NADPH in MT-2 cells was...
After overnight incubation, on day 5, the medium and the compounds were removed from the cells and 20 µL of 1:20 dilution of BrightGlo (Promega) in Tango assay buffer supplemented with 0.01% pluronic F68 was added to the cells. Plates were incubated for 20 minutes in the dark ...
Impact of GLO1 Knock Down on GLUT4 Trafficking and Glucose Uptake in L6 Myoblasts DOI: 10.1371/journal.pone.0065195 Britta Engelbrecht, Bernd Stratmann, Cornelius Hess, Diethelm Tschoepe, Thomas Gawlowski Full-Text Cite this paper Add to My Lib Abstract: Methylglyoxal (MG), a highly reactive...
Caspase 3/7 activity assay. Caspase 3/7 activity was measured using caspase-Glo 3/7 assay kit (Promega). Equal number of live cells from different treatment conditions was used for the assay. Cells were incubated with substrates for 1 h at room temperature and a luminescent signal was ...
Fig. 7: Decreased GLUT2 expression, glucose uptake and ATP production in MODY3 mutant β-like cells. aGlucose uptake in WT (red) and patient-specific (blue) hPSC-derived β-like cells.n = 3 independent experiments;p = 0.0182 (P1), 0.0294 (P2).bATP: ADP ratio in WT (red)...