Glucose uptake The non-radioactive 2-dexoyglucose uptake was detected using a Glucose Uptake-GloTM Assay Kit (J1341, Promega, Madison, USA) according to manufactured instruction. Briefly, cells were seed on 96-well plates and serum starved for 3 h before the assay. Cells were washed with...
Glucose uptake MIN6 cells were cultured in 24-well plates (8 × 104 cells/well) for 3 d. The cells were treated as indicated. The glucose uptake assay was performed following the Glucose Uptake-Glo Assay manufacturer’s instructions. The medium was removed and washed with 500 μl of...
The Glutamate-GloTM assay kit (Promega, J7021) was used to measure intracellular glutamate levels. Briefly, the cells were seeded in a 96-well plate, followed by glucose starvation as described above. Cells were then washed with ice-cold PBS and lysed with 0.6 n HCl. The lysing reagent wa...
The CR way[TM] to great glucose control.(Report)BMcGlothin, PaulAverill, Meredith
LPD treatment reduced Aβ plaques and increased brain glucose uptake in a PPARγ-dependent manner. The brain sections (n = 3 per group) were stained by immunofluorescence using an anti-Aβ antibody. A Immunofluorescence images of Aβ plaques in the hippocampus. Scale bar = 200 μm. Arrows ...
Decreased GLUT2, glucose uptake and ATP production To determine if theHNF1A+/H126Dmutation had any effects on glucose uptake into the β-like cells, we compared the glucose uptake between WT and mutant β-like cells. Interestingly, the glucose uptake inHNF1A+/H126Dmutant β-like cells from...
Since GLP-1-induced insulin secretion requires glucose metabolism, we aimed to address the hypothesis that GLP-1 receptor (GLP-1R) signalling can modulate glucose uptake and utilization in β-cells. We have assessed various metabolic parameters after short and long exposure of clonal BRIN-BD11 β...
we identified 20-HETE as a new regulator of pancreatic β-cells. 20-HETE turned out to be a highly efficacious agonist of FFAR1, which is produced by PLA2and ω-hydroxylases after uptake of glucose by β-cells. Inhibition of 20-HETE formation as well as blockade of the FFAR1 receptor ...
it was suggested that SOCS3 served as a bridge between immune response and glucose metabolism. IL-6 increased the gene expression ofSOCS3and thus regulated the downstream targets of PI3K-AKT mediated signal transduction that underlie the downregulation ofGLUT2activity, glucose uptake and metabolism in...
Glucose uptake was measured using Glucose Uptake-Glo Assay (Promega, MI, USA) in accordance with the manufacturer's instructions. 2.9. Immunofluorescence Analysis for GLUT4 Localization Immunofluorescence staining for GLUT4 was performed as previously described [5]. Briefly, mouse muscles were dissected...