Glucose uptake The non-radioactive 2-dexoyglucose uptake was detected using a Glucose Uptake-GloTM Assay Kit (J1341, Promega, Madison, USA) according to manufactured instruction. Briefly, cells were seed on 96-well plates and serum starved for 3 h before the assay. Cells were washed with...
Glucose Uptake-Glo Assay Kit Progema Cat#J1341 Lactate Colorimetric/Fluorometric Assay Kit Biovision Cat#K607-100 BCA protein assay Kit Beyotime Cat#No.P0012 Deposited data Unprocessed blots and gels This paper https://doi.org/10.17632/9xwvvvcsz6.1 RNA-seq data This paper GSE206393 and GSE206...
Since GLP-1-induced insulin secretion requires glucose metabolism, we aimed to address the hypothesis that GLP-1 receptor (GLP-1R) signalling can modulate glucose uptake and utilization in β-cells. We have assessed various metabolic parameters after short and long exposure of clonal BRIN-BD11 β...
The Glutamate-GloTM assay kit (Promega, J7021) was used to measure intracellular glutamate levels. Briefly, the cells were seeded in a 96-well plate, followed by glucose starvation as described above. Cells were then washed with ice-cold PBS and lysed with 0.6 n HCl. The lysing reagent wa...
The CR way[TM] to great glucose control.(Report)BMcGlothin, PaulAverill, Meredith
Glucose uptake assay C2C12 and HepG2 cells at 80% confluence in 100 mm petri dishes were pre-treated with either 0.3 mM ALA or vehicle for 8 h and then, the media was replaced with glucose free media, containing either ALA or vehicle, for overnight starvation. The next day, the culture...
Cells were then washed with Cell-Based Assay Buffer and transferred to 96-well assay plate at 1 × 104 cells/well. Finally, fluorescence was quantified on Glomax Multi+ Microplate Multimode Reader (Promega, Madison, WI) as previously described29,30. Monitoring glucose uptake in C2C12 and...
Decreased GLUT2, glucose uptake and ATP production To determine if theHNF1A+/H126Dmutation had any effects on glucose uptake into the β-like cells, we compared the glucose uptake between WT and mutant β-like cells. Interestingly, the glucose uptake inHNF1A+/H126Dmutant β-like cells from...
However, as enhanced glucose uptake can outstrip microenvironment supplies, the growing tumor has to cope with a potentially lethal metabolic stress until it is partly relieved by angiogenesis (Gillies et al., 2008; Jones and Thompson, 2009). The molecular mechanisms that allow tumor cell survival...
it was suggested that SOCS3 served as a bridge between immune response and glucose metabolism. IL-6 increased the gene expression ofSOCS3and thus regulated the downstream targets of PI3K-AKT mediated signal transduction that underlie the downregulation ofGLUT2activity, glucose uptake and metabolism in...