Adipocyte glucose uptake was measured using the Glucose Uptake Assay Kit (ab136955, Abcam, Cambridge, UK) as described in the ESM Methods.Mitochondrial respiratory functionMitochondrial respiratory function was assessed using the Seahorse XFp Extracellular Flux Analyzer (Seahorse Bioscience, Agilent ...
Glucose uptake Glucose uptake was measured with the Glucose Uptake Assay Kit (Abcam, Cambridge, UK). Cells were seeded in a 96-well plate at a density of 2,000 cells/well and were starved in 100 μL of serum-free medium overnight to increase glucose uptake. 2-Deoxyglucose (2-DG) was...
Cells were (1.0 × 105) were incubated overnight on 6-well plates, intracellular lactate and ATP production were determined with Lactate Colorimetric/Fluorometric Assay Kit (Biovision, Abcam) and ATP Bioluminescence Assay Kit CLS II (Roche Applied Science) according to the manufacturer’s pro...
These findings suggest that TNBC cells inhibit PI3K/AKT/mTOR signaling pathway in CD8 + T cells, which resulted in reduced glucose uptake and lactate production. Furthermore, our research revealed that blocking TIGIT led to the activation of metabolic pathways in CD8 + T cells. ...
2-NBD-Glucose试剂CAS号:186689-07-6保存条件:-20°C产品介绍:Glucose uptake probe上海惠诚生物为广大科研用户提供科学仪器,试剂耗材等专业科研产品服务。 代理产品品牌有:Nu-chek、LKT、AccuStandard、APSC、MPBio、Sigma-Aldrich、NIST,Reagecon、Megazyme,LGC、Ultra,日本和光(WAKO)、Shodex,Dr.E、PSS ,Matreya,...
Here, we examine the role of glucose uptake and the glucose transporter Glut1 in the metabolism and metabolic stress response of BCR-Abl+ B-cell acute lymphoblastic leukemia cells (B-ALL). B-ALL cells were highly glycolytic and primary human B-ALL samples were dependent on glycolysis. We ...
For glucose uptake assay, cells were seeded in six-well plate, and then treated with 30 nM 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2- Deoxyglucose (2-NBDG) for 1 h at 37 °C. The uptake of 2-NBDG was analysed by flow cytometry. To measure glucose output, cells were...
(GH) and insulin-like growth factor-1 (IGF1) (J) of P-BCA Sera and P-BCA SV Cell-Conditioned Media Increase Glucose Uptake in Wild-Type Muscle Explants To further discriminate between intrinsic and extrinsic differ- ences in P-BCA muscles, we turned to an ex vivo glucose uptake assay....
Given the remaining capacity for glucose uptake in Glut1-deficient cells, the metabolic impact of Glut1 deletion was unclear. To better understand the metabolic role of Glut1, the steady-state levels of 410 metabolites were measured through non-targeted LC/MS22 in B-ALL cells, with or without...
Pancreatic β cell dysfunction contributes to the pathogenesis of type 2 diabetes. MiR-21 has been shown to be induced in the islets of glucose intolerant patients and type 2 diabetic mice. However, the role of miR-21 in the regulation of pancreatic β c