glucoseuptakecolorimetricassaykitabcam ProductnameGlucoseUptakeAssayKit(Colorimetric) DetectionmethodColorimetric SampletypeAdherentcells,Suspensioncells AssaytypeCell-based(quantitative) Sensitivity<=0.01nmol/well SpeciesreactivityReactswith:Speciesindependent Productoverview GlucoseUptakeAssayKit(Colorimetric)(ab136955)is...
A Cell viabilities were determined by CCK8 assay 72 h after drug treatment. B Apoptosis was analyzed 48 h after drug treatment. C 2-NBDG for glucose uptake was measured 24 h after drug treatment. D–F The primary CML cells (#1, #2, #3) from patients were cultured and treated...
Adipocyte glucose uptake was measured using the Glucose Uptake Assay Kit (ab136955, Abcam, Cambridge, UK) as described in the ESM Methods.Mitochondrial respiratory functionMitochondrial respiratory function was assessed using the Seahorse XFp Extracellular Flux Analyzer (Seahorse Bioscience, Agilent ...
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The glucose consumption of colon cancer cells was measured using the glucose uptake assay kit (#ab136955, Abcam, Shanghai, China) according to the manufacturer’s instruction. The ECAR was detected by the Seahorse XF glycolysis stress test kit from Agilent Technologies using the Seahorse XFp analy...
Chronic BCAA supplementation has been shown to induce insulin resistance in animal models primarily by lowering insulin-stimulated glucose uptake [21, 63]. The proposed mechanisms include hyperactivation of mTOR [21], reduction of AKT by its ubiquitination [63], and excess accumulation of valine ...
ATP levels in hepatic and adipose tissue homogenates were estimated calorimetrically using ATP assay kit (Abcam, ab83355) as per manufacturer's instructions. Hematoxylin and Eosin (HE) staining. Formalin fixed tissues were processed in graded concentrations of ethanol followed by xylene prior to ...
These findings suggest that TNBC cells inhibit PI3K/AKT/mTOR signaling pathway in CD8 + T cells, which resulted in reduced glucose uptake and lactate production. Furthermore, our research revealed that blocking TIGIT led to the activation of metabolic pathways in CD8 + T cells. ...
Glucose uptake MIN6 cells were cultured in 24-well plates (8 × 104 cells/well) for 3 d. The cells were treated as indicated. The glucose uptake assay was performed following the Glucose Uptake-Glo Assay manufacturer’s instructions. The medium was removed and washed with 500 μl of...
Pancreatic β cell dysfunction contributes to the pathogenesis of type 2 diabetes. MiR-21 has been shown to be induced in the islets of glucose intolerant patients and type 2 diabetic mice. However, the role of miR-21 in the regulation of pancreatic β c