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The glucose concentration in the tissue digestion solution was measured using the Glucose (GO) Assay Kit (Sigma-Aldrich, St. Louis, Mo) according to manufacturer’s instruction. Briefly, 50 µL of the tissue digestion solution were mixed with 100 µL of the assay reagent and incubated...
Absolute quantification of ATP, ADP, AMP and α-ketoglutarate was obtained by comparison to a calibration curve of pure standards (Sigma-Aldrich, St Louis, MO, USA). For analysis of fatty alcohols, 500 μl of cell culture was extracted with 500 μl of 2:1 (v/v) chloroform/methanol ...
货品编号:Sigma.g7021-1kg 数量:-+支 正品货源 质量保障 商品详情 商品评论 商品咨询 品牌:Sigma-Aldrich 所属分类:生化标准品 货品编号: Sigma.g7021-1kg 品牌:Sigma 品名:D-(+)-葡萄糖 D-(+)-GLUCOSE, POWDER, BIOREAGENT, SUITA 规格:1kg ...
Glucose production by mouse primary hepatocytes was measured according to the manufacturer's protocol using a glucose oxidase assay (Sigma-Aldrich, St Louis, MO, USA). Briefly, the cells were incubated for 6 h at 37 °C and 5% CO2 in the Krebs-Ringer buffer (115 mM NaCl, 5.9 mM KCl, ...
Two days after transfer, mice were treated with vehicle (corn oil; Sigma- Aldrich) or tamoxifen (120 mg/kg per day; Sigma-Aldrich) by i.p. injection for four consecutive days. GFP+B-ALL cells were observed in spleen and bone marrow by flow cytometry. In some cases, animals were ...
The supernatant of cell lysates was collected by centrifugation at 12,000 rpm for 15 min and the protein concentration was determined by Bradford assays (Cat. No. B6916, Sigma-Aldrich, Inc). Total protein of each sample was separated by 10% SDS-PAGE gel and then transferred to a ...
GC cell lines, SGC7901 cells (mutant-type of p53), and cisplatin-resistance cells (SGC7901CR) were obtained from and STR identified by KeyGENE Bio Co. Ltd (Nanjing, China). Cisplatin (Pt(NH3)2Cl2, >99.0% purity), were purchased from Sigma-Aldrich (Shanghai, China). Cells were culture...
Sigma-Aldrich G7021 D-(+)-Glucose Quick View Properties product line EX-CELL® Quality Level 500 form dry powder technique(s) cell culture | mammalian: suitable (suspension) shipped in ambient storage temp. 2-8°C Looking for similar products? Visit Product Comparison Guide ...