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The glucose concentration in the tissue digestion solution was measured using the Glucose (GO) Assay Kit (Sigma-Aldrich, St. Louis, Mo) according to manufacturer’s instruction. Briefly, 50 µL of the tissue digestion solution were mixed with 100 µL of the assay reagent and incubated...
货品编号:Sigma.g7021-1kg 数量:-+支 正品货源 质量保障 商品详情 商品评论 商品咨询 品牌:Sigma-Aldrich 所属分类:生化标准品 货品编号: Sigma.g7021-1kg 品牌:Sigma 品名:D-(+)-葡萄糖 D-(+)-GLUCOSE, POWDER, BIOREAGENT, SUITA 规格:1kg ...
The supernatant of cell lysates was collected by centrifugation at 12,000 rpm for 15 min and the protein concentration was determined by Bradford assays (Cat. No. B6916, Sigma-Aldrich, Inc). Total protein of each sample was separated by 10% SDS-PAGE gel and then transferred to a ...
Glucose production by mouse primary hepatocytes was measured according to the manufacturer's protocol using a glucose oxidase assay (Sigma-Aldrich, St Louis, MO, USA). Briefly, the cells were incubated for 6 h at 37 °C and 5% CO2 in the Krebs-Ringer buffer (115 mM NaCl, 5.9 mM KCl, ...
GC cell lines, SGC7901 cells (mutant-type of p53), and cisplatin-resistance cells (SGC7901CR) were obtained from and STR identified by KeyGENE Bio Co. Ltd (Nanjing, China). Cisplatin (Pt(NH3)2Cl2, >99.0% purity), were purchased from Sigma-Aldrich (Shanghai, China). Cells were culture...
(C) GO analysis of the 91 proteins enriched in both the dextran column and azido glucose purification. (D) qPCR of progenitor markers in progenitor cells after DDX21 knockdown, normalized to scrambled shRNA control. (E) CTB assay of the cell proliferation rate of keratinocyte progenitor cells ...
The cells were cultured in RPMI 1640 medium (Sigma- the cell lines (see above). Aldrich Sweden AB, Stockholm, Sweden) (containing 25 mM Hepes for the RS4 and SupB15 cell lines), supplemented with 10% (v/v) heat-inactivated fetal calf serum, 2 mM L-glutamine, 50 mg/ml streptomycin...
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(S)) according to the provider’s instructions. Formal consent by the donors and ethical approval was obtained. Insulin secretion assays were performed on the second day after arrival of islets using a human insulin ELISA kit from Sigma-Aldrich and the same protocol as described for murine ...