品牌:Sigma-Aldrich 优惠价:立即咨询 产品价格 产品编号包装单位单价(元)国内现货国外库存询价单 30917021 KIT8620 产品别名 糖原检测试剂盒 Glycogen Assay Kit 基本信息 NACRES NA.84 General description【一般描述】 糖原是一种葡萄糖支链聚合物,是动物体内主要短期能量储存分子。 糖原主要在肝脏和肌肉组织中合成,占...
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For glycogen quantification, the MAK016 assay kit (Sigma-Aldrich) was used. 25 µl of appropriate dilutions of the samples were mixed with hydrolysis buffer and further processed according to the manufacturer’s instruction. Western blotting For the detection of FLAG-tagged GlgX, 5–10 ...
Recombinant human tumor necrosis factor (TNF)-α and monocyte chemoattractant protein 1/chemokine ligand (CCL)2 were obtained from R&D Systems (Minneapolis, MN). GSK3β inhibitors were obtained as follows: lithium chloride (LiCl; Sigma/Aldrich Co, Ltd, St Louis, MO), 5-iodo-indirubin-3′-mo...
Glycogen concentration assay Glycogen was quantified using a coupled enzyme colorimetric Glycogen Assay Kit (Sigma-Aldrich, UK) according to manufacturer’s protocol, normalized to protein concentration [Pierce BCA Protein Assay Kit (Thermo Fisher, UK)]. ...
To confirm that oxidants do, indeed, deplete glycogen, we monitored the glycogen level in worm cells lysates using an enzymatic assay or in live animals by iodine staining (Fig. 4dandSupplementary Fig. 4b). Glucose-fed worms accumulated twofold more glycogen than worms fed a regular diet (Fig...
EXPERIMENTAL PROCEDURES RESULTS DISCUSSION Acknowledgments ReferencesFigures (8) Show 2 more figuresTables (1) Genes, primers, and conditions for semiquantitative PCR amplificationJournal of Biological Chemistry Volume 281, Issue 21, 26 May 2006, Pages 14841-14851 Mechanisms of Signal Transduction...
Concentration of intracellular glycogen was analysed using Glycogen Colorimetric Assay Kit II (BioVision) and was compared between TYNEK-3 and ESCC cell lines. TE-5, TE-8 and TE-10 cells were treated for 24 h with dimethyl sulfoxide (DMSO; Sigma-Aldrich) or with one of the GSK3β inhibit...