品牌:Sigma-Aldrich 优惠价:立即咨询 产品价格 产品编号包装单位单价(元)国内现货国外库存询价单 30917021 KIT8620 产品别名 糖原检测试剂盒 Glycogen Assay Kit 基本信息 NACRES NA.84 General description【一般描述】 糖原是一种葡萄糖支链聚合物,是动物体内主要短期能量储存分子。 糖原主要在肝脏和肌肉组织中合成,占...
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(v/v) ethanol. The pellet was dried using a speed-vac for 20 min at 60 °C and finally resuspended in 200 µl PBS by shaking at rt for 2 h at 600 rpm. For glycogen quantification, the MAK016 assay kit (Sigma-Aldrich) was used. 25 µl of appropriate dilutions ...
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DAB (2 μL, 300 pmol, D1542, Sigma-Aldrich) or saline was injected into the lateral ventricle through a cannula (62003, RWD Life Science) placed 1 week before the experiment (0.22 mm posterior to bregma, 1.0 mm lateral, 1.5 mm deep). Analysis of Anesthesia Induction and Emergence Each ...
Glucose liberated after enzymatic hydrolysis of glycogen was determined by a colorimetric method, using a glucose assay kit (Sigma). A blank of the reaction was performed by incubating SR membranes without amyloglucosidase. A calibration curve was constructed with known amounts of glycogen. Assay of...
The NADH generation was assessed by an enzyme-coupled assays kit (Hexokinase Colorimetric Assay Kit: Bio Vision Inc, USA) and a multi plate reader Synergy™ HT (Biotek Instruments) with the measurement of absorbance at 450 nm in 96-well plates according to the manufacturer’s protocol. b ...
All enzymes added to the buffer for coupled enzymatic assays were purchased from Sigma-Aldrich and prepared from rabbit muscle. In all assay systems, activity was detected with and without addition of substrate to subtract unspecific background. Hexokinase activity was assayed as NADP reduction in ...