Glucose(GO)AssayKitProductCodeGAGO-20TECHNICALBULLETINProductDescriptionEnzymes,asanalyticaltools,havefoundwides..
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The glucose concentration in the tissue digestion solution was measured using the Glucose (GO) Assay Kit (Sigma-Aldrich, St. Louis, Mo) according to manufacturer’s instruction. Briefly, 50 µL of the tissue digestion solution were mixed with 100 µL of the assay reagent and incubated...
Relative glucose production (e) was measured using a glucose oxidase (GO) kit. Data are represented as mean ±s.d., N ¼ 5 for each group. *Po0.05, **Po0.01, versus CRY1 þ / þ control (Student's t-test). (f) Mouse primary hepatocytes were infected with Ad-MOCK and Ad-...
Analytical methods Extracellular glucose was measured using an enzymatic assay (GOPOD kit, Megazyme, Bray, Ireland). Extracellular acetate, lactate and pyruvate were measured by high pressure liquid chromatography with a UV absorbance detector (Agilent, Santa Clara, CA, USA). For growing cells, ...
The human fungal pathogen Candida albicans can switch stochastically and heritably between a “white” phase and an “opaque” phase. Opaque cells are the mating-competent form of the species, whereas white cells are thought to be essentially “sterile”
Click-&-Go Click Chemistry Capture Kit Click Chemistry Tools #1065 MagReSyn Streptavidin beads Resyn Biosciences MR-STV010 BS3 (bis(sulfosuccinimidyl)suberate) ThermoFisher #21580 LDS sample loading buffer Invitrogen NP0008 Trypsin/Lys-C Promega V5071 Iodoacetamide Sigma I1149-5G Dithiothreitol MP ...
(e) was measured using a glucose oxidase (GO) kit. Data are represented as mean ±s.d.,N=5 for each group. *P<0.05, **P<0.01, versusCRY1+/+control (Student’st-test). (f) Mouse primary hepatocytes were infected with Ad-MOCK and Ad-CRY1, and then treated with insulin (10 ...
(Accutrol, Sigma No. 2034) was run in each plate to assure accuracy. TC was determined according to Allain et al.69, as provided in a kit (Sigma procedure 352). HDL-C was separated from LDL-C + VLDL-C by precipitating the latter with phosphotungstic acid and magnesium chloride acc...
21 Gozuacik D, Kimchi A. Autophagy and cell death. Curr Top Dev Biol 2007; 78: 217–245. This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http:// creativecommons.org/licenses/by-nc...