近日，重庆市第三军医科大学细胞生物学系研究团队在国际期刊杂志SCIENCE ADVANCES上发表题为“Engineered exosomes reprogram Gli1+ cells in vivo to prevent calcification of vascular grafts and autologous pathological vessels”的综述，阐释了工程外泌体在体内重新编程Gli1+细胞，以防止血管移植物和自体病理血管钙化。
胆管反应(Ductular Reaction,DR)是肝脏疾病中常见的病理变化,主要表现为在肝脏发生病变时,肝组织内伸长、扁薄、无管腔的不典型胆管样结构的细胞数量增加。在人类和啮齿类动物肝脏中,DR主要有三种类型:一是固有胆管增生,二是肝细胞的胆管化生,...
Here, we show that Gli1 cells, which are a subpopulation of MSCs couple with and regulate a specialized form of vasculature. The specific capillaries, i.e., CD31hiEMCNhi type H vessels, are the preferable vascular subtype which Gli1 cells are adjacent to in bone. Gli1 cells are further ...
近日,重庆市第三军医科大学细胞生物学系研究团队在国际期刊杂志SCIENCE ADVANCES上发表题为“Engineered exosomes reprogram Gli1+ cells in vivo to prevent calcification of vascular grafts and autologous pathological vessels”的综述,阐释了工程外泌体在体内重新编程Gli1+细胞,以防止血管移植物和自体病理血管钙化。
肌肉干细胞 (muscle stem cells, MuSCs) 对骨骼肌的损伤修复起到决定性作用。良好的骨骼肌再生能力可以维持肌肉稳态、促进损伤修复；反之，再生障碍将导致肌肉萎缩和功能减退。局部损伤和环境应激可导致MuSCs向中间状态转化，被称为GAlert状态。这种状态可以使MuSCs更快地进入细胞周期并更有效地分化。GAlert MuSCs被认为...
肌肉干细胞(muscle stem cells,MuSCs)对骨骼肌的损伤修复起到决定性作用。良好的骨骼肌再生能力可以维持肌肉稳态、促进损伤修复;反之,再生障碍将导致肌肉萎缩和功能减退。局部损伤和环境应激可导致MuSCs向中间状态转化,被称为GAlert状态。这种状态可以使MuSCs更快地进入细胞周期并更有效地分化。GAlert MuSCs被认为是肌肉...
Western blot analysis of extracts from RMS-13 and TOV-112D cells using GLI1 (V812) Antibody. 1/1 Learn more about how we get our images To Purchase # 2534 Cat. # Size Price Inventory 2534T 20 µl ¥1,650 库存充足 2534S 100 µl ¥4,550 库存紧张 REQUEST A PRICE...
GLI1 RNA的选择性剪接可导致外显子I-III的缺失,在n端共计128个氨基酸,形成GLI1∆N变体。C'∆GLI1和N'∆GLI1变异被认为分别来自于全长GLI1基因产物的翻译后c端和n端截断。外显子III和部分外显子IV的缺失,总计41个氨基酸,产生tGLI1亚型。图片源自于doi:10.3390/cells9092114。
GLI was first identified as a gene amplified in a malignant glioma (1) capable of transforming primary cells in cooperation with adenovirus E1A (2). GLI belongs to the Krüppel family of zinc finger proteins that includes three mammalian GLI proteins: GLI1, GLI2, and GLI3 (3). These GLI...
(2)容易实现高密度培养:理论培养密度是1×1013 cells/ml,实际培养过程中使用LB培养基在37℃培养大肠杆菌,<1×1010 cells/ml。在低温(11℃)诱导蛋白表达时,细菌个数几乎不增长。 (3)转染外源DNA容易,质粒转染效率高。 GLI家族锌指蛋白1(GLI1)重组蛋白问题2:表达质粒体系选择: ...