Antibody against Glc7 (1:5000) was custom-made in Abclonal and its specificity was confirmed by dot blots with Glc7 peptide (Supplementary Fig. S11b). H3pT11 was validated for ChIP assay in H3T11A mutant and PKM2 knockdown HUVECs (Supplementary Fig. S11c, d). Chromatin immunoprecipitation...
Only two of these PPases are clearly essential: the PP1-type PPase, Glc7 (gl yc ogen deficient) [1, 22], and Cdc14 (c ell d ivision c ycle), a dual-specificity phosphatase that regulates mitotic/meiotic exit [23]. We selected Glc7 for our analyses since a framework of genetic ...
1989). Using extracts from Myc-tagged Glc7p containing cells as a negative control for immunoprecipitation with anti-HA antibody, no H3 phosphatase activity was detected in the immunoprecipitants. Finally, gel analyses showed that the substrates were not degraded and Western blot confirmed that the...
Immunoprecipitation with the anti-T antibody HH8 (lane 2) leads to a partial overlap of precipitated proteins, whereas a nonrelevant IgM antibody (lane 3) did not reveal comparable protein enrichment in the respective mass range, indicating that most of the immunoprecipitated protein in Figure 4FS...