How cells adapt their gene expression to nutritional changes remains poorly understood. Histone H3T11 is phosphorylated by pyruvate kinase to repress gene transcription. Here, we identify the protein phosphatase 1 (PP1), Glc7 as the enzyme that specifica
Rett syndrome (RTT), a neurodevelopmental disorder affecting exclusively (99%) female infants, is associated with loss-of-function mutations in the gene encoding methyl-CpG binding protein 2 (MECP2) and, more rarely, cyclin-dependent kinase-like 5 (CDKL5) and forkhead box protein Gl (FOXG1...
Protein kinases and phosphatases regulate protein phosphorylation, a critical means of modulating protein function, stability and localization. The identification of functional networks for protein phosphatases has been slow due to their redundant nature
1989). Using extracts from Myc-tagged Glc7p containing cells as a negative control for immunoprecipitation with anti-HA antibody, no H3 phosphatase activity was detected in the immunoprecipitants. Finally, gel analyses showed that the substrates were not degraded and Western blot confirmed that the...
Rett syndrome (RTT), a neurodevelopmental disorder affecting exclusively (99%) female infants, is associated with loss-of-function mutations in the gene encoding methyl-CpG binding protein 2 (MECP2) and, more rarely, cyclin-dependent kinase-like 5 (CDKL5) and forkhead box protein G1 (FOXG1...
Immunoprecipitation with the anti-T antibody HH8 (lane 2) leads to a partial overlap of precipitated proteins, whereas a nonrelevant IgM antibody (lane 3) did not reveal comparable protein enrichment in the respective mass range, indicating that most of the immunoprecipitated protein in Figure 4FS...