2.根据权利要求1所述的一种靶向抑制绿色荧光蛋白GFP基因表达的siRNA序列,其特征在于:所述siRNA的克隆载体为pSilencer2.1-U6neo。 3.根据权利要求2所述的一种靶向抑制绿色荧光蛋白GFP基因表达的siRNA序列,其特征在于:所述的pSilencer2.1-U6neo长度为4521bp。 4.根据权利要求3所述的一种靶向抑制绿色荧光蛋白GFP基因表...
1.寡核苷酸单链的设计及合成 采用Ambion公司的siRNATargetFinder(onlinetargetfinder)进行siRNA的设计(http://www.ambion.com/techlib/misc/siRNA_finder.html),公司合成。 siRNATargetSequenceONE:5'-AAGGTGATGCTACATACGGAA-3' SensesiRNAstrand:5'-GGUGAUGCUACAUACGGAAtt-3' AntisensesiRNAstrand:3'-UUCCGUAUGUAGCA...
Two pairs of oligonucleotides were synthesized according to the Tuschl principle and inserted into plasmid pGenSil-I to generate siRNA eukaryotic expression vector. DH5α strains were transformed, plasmids were extracted, and recombinant vectors were identified by the restriction map and the sequence ...
1.寡核苷酸单链的设计及合成 采用Ambion公司的siRNATargetFinder(onlinetargetfinder)进行siRNA的设计(https://www.ambion.com/techlib/misc/siRNA_finder.html),公司合成。 siRNATargetSequenceONE:5'-AAGGTGATGCTACATACGGAA-3' SensesiRNAstrand:5'-GGUGAUGCUACAUACGGAAtt-3' AntisensesiRNAstrand:3'-UUCCGUAUGUAGC...
With these off-target effects in mind, we used a commonly used siRNA sequence directed againstGFPin a series of microarray expression profiling experiments, in which we knocked down four unrelated candidate suppressor genes (manuscript in preparation). We carefully analyzed the resulting data set and...
11、XA,进行逆转录PCR反映,结果如图4示,干扰组(GFP/RNAi组)由于RNAi的干扰作用,使Bel2cDA表达量降低(图4A),而参照基因GAPDH的eDNA表达量并无发生明显的转变(图4B),说明所设计的siRNA载体能有效的抑制目的基因Bel2cDXA的表达。1 Bel2表达分析,B.GAPDH表达分析;1:GFP/RNAigroup(干扰组),2:GFP/Controlgroup(对...
ThesiRNAcansilencethemRNAofhomologousgenesequencespeciallyand efficientlywithoutinfluencingthefunctionofothergene,itisanewgenetictherapyto geneticdisease,tumorandvirusinfection.ThisthesisadoptssiRNA/liposomesto transfecthepatocarcinomacell(HepG2)tosilencetheexpressionoftargetgene(GFP) ...
pCDH-MSCV-MCS-EF1-copGFP-T2A-Puro pCDH-MSCV-MCS-EF1-copGFP-T2A-Puro载体基本信息：载体名称: pCDH-MSCV-MCS-EF1-copGFP-T2A-Puro 质粒类型: 慢病毒表达载体；cDNA表达载体；双启动子载体克隆方法: 多克隆位点，限制性内切酶 启动子: MSCV 载体大小: -- 5' 测序引物及序列: MSCV: GGGGTACAGTGCAGGGGAAA...
siRNA mediated knockdown. HeLa cells were maintained in culture medium. Knockdowns were per- formed by treating cells with 5 nM total siRNA (Qiagen), using the Hiperfect transfection reagent, accord- ing to the manufacturer's instructions for fast-forward transfection (Qiagen). The All-...
Product Category: Pre-made recombinant lentivirus; LV-EF1α-NLS-GFP-Puro; LV-EF1A-NLS-GFP-Puro; EF1α-NLS-GFP-Puro Lentivirus; EF1A-NLS-GFP-Puro Lentivirus Description: LV-EF1α-NLS-GFP-Puro is a pre-made lentivirus which express eGFP fused with SV40 nuclear localization sequence (NLS) ...