The green fluorescent protein (GFP) was originally isolated from the Jellyfish Aequorea Victoria that fluoresces green when exposed to blue light. GFP protein is composed of 238 amino acids with the molecular mass of 26.9 kD. The GFP gene is frequently used in cellular and molecular biology as...
Description: This GF28R monoclonal antibody reacts with green-fluorescent protein (GFP), which was originally isolated from the cnidarian Aequorea victoria. This 27-kDa protein absorbs blue light (maximally at 395 nm) and emits green light (peak at 509) without the requirement of exogenous substr...
In A.victoria, the 27-kDa protein absorbs blue light from a photoprotein that is activated by calcium and emits green light (2). GFP was isolated from A. victoria and the cDNA was originally cloned in 1992 by Douglas Prasher (1). Genetic manipulations of GFP has made it possible for ...
Green fluorescent protein (GFP) is a 27 kDa protein originally isolated from the jellyfishAequoreavictoria. In the presence of UV light (490-520 nm), it emits a green fluorescent color that can be used to pinpoint locations of various intracellular proteins. GFP is 238 amino acids (aa) in...
Interestingly, both mutants were thermosensitive and at 30°C sgGFP-Δ D130 was more fluorescent than the parent protein. In contrast with deletions, substitutions of single amino acids from residues F131 to L142 were well tolerated. The substitution analysis revealed a particular importance of ...
as a reporter gene has greatly advanced the study of gene expression, protein localization, and cell and tissue development in a multitude of disciplines. gfp, an intrinsically fluorescent protein originally isolated from the jell...
英文名称:Green Fluorescent Protein (GFP) 品牌:Solarbio 分子式:现询客服 货号:G7950 产地:北京 纯度:现询客服 % 是否进口:否 Protein Description The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. GFPSparkTM is an improved variant of...
2.3. Basal activity of PT7lacpromotesM. tuberculosismembrane protein expression inMycobacterium smegmatismc24517 The frGFP fusion technique was originally developed for soluble and membrane protein folding and expression screening inE. coli[12,22]. Also, it was shown to be applicable for protein expre...
PVDF membrane was probed with 1 µg/mL of Goat Anti-GFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4240) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GFP at approximately 80 kDa (as indicated). This ...
GFP was detected in 2% paraformaldehyde immersion fixed HEK293 human embryonic kidney cell line transfected with GFP-expressing protein using Mouse Anti-GFP Monoclonal Antibody (Catalog # MAB42401) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557...