These leads to a strong stabilization of the GFP molecule, leading to a) a strong nuclear signal and b) retention of fluorescence even after the cells no longer express Pdgfra. In the brain of this reporter mouse, the majority of cells expressing GFP are of the oligodendrocyte lineage. ...
copGFP质粒标记的阳性细胞
cells (H9Zn2.3GFP), with the goal of marking the cells that have become differentiated osteoblasts. In teratomas formed using these cells, we identified green fluorescent protein (GFP)-positive cells specifically associated with in vivo bone formation. We also differentiated the cells into a ...
GFP positive cells can be observed on Day 5 (Figure 2). Figure 1 | Sox1-GFP mESCs express typical ES cell markers Figure 2 | Monoculture neuronal differentiation of Sox1-GFP mESCs on Day 5 Growth Medium Embryonic stem cell medium DMEM: 415ml FBS: 75ml (HYCLONE LABORATORIES INC, Cat# ...
GFP positive cells express pericyte markers.doi:10.1371/journal.pone.0123553.g005Svensson AndreasIlknurGenové GuillemPaul GesineB. JohanPLOS ONE
1 Cells are selected from THP-1 cells transfected with GFP-SV40 expressing lentiviruses resistant to Blasticidin, and sorted by flowcytometery for GFP positive cells and cloning. The cells are shipped in frozen vials with > 5 x 105 cells/vial. GFP-THP-1 cells are with THP-1 cultured ...
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10、stable hela cells expressing egfp-lc3 with an autophagy-inducing small molecule increases the formation of autophagic vesicles (egfp-positive punctate structures). sarkar et al, nat chem biol, 3(6):331-338 (2007).27发育生物学long term 4d imaging of caenorhabditis elegans embryo28神经生物学29...
the cells with propidium iodide we can monitor the viability of the culture during GFP expression. •In cultures that are co-transduced with GFP and RFP, the Cellometer has the capability to capture, analyze, and report the population of GFP positive, RFP positive,...
After infection with AdEgr-GFP, MM45T.Li tumor cells were exposed to different doses of γ-irradiation from 0 Gy to 15 Gy in vitro. The percentage of GFP expression positive cells increased greatly in a dose-dependent manner as detected by FACS and Western blot analysis. For in vivo ...