其他还包括发射荧光也发生改变的变体,包括蓝色荧光蛋白:EBFP,EBFP2,Azurite,mKalama;青色荧光蛋白:ECFP,Cerulean,CyPet;黄色荧光蛋白:YFP,Citrine,Venus,YPet等等。 【GFP的激发光波长和GFP的发射光波长】链接地址:https://www.luyoruv.com/news/news2/GFP-Excitation-Emission.html 未经公司同意,严禁转载我站文章,...
Excitation (激发波长, Emission (发射波长, nm ) nm ) Fluorescent Dye (荧光 染料) Cy2 489 506 GFP(Red Shifted) 488 507 YO-PRO -1 491 509 YOYO -1 491 509 Calcein 494 517 FITC 494 518 FluorX 494 519 Alexa 488 490 520 Rhodamine 110 496 520 ABI,5-FAM 494 522 Oregon Green ?500 50...
Photo conversion Any method that enables excitation of, or emission from, GFP at an alternative wavelength may be useful in avoiding areas of the spectrum where autofluores- cence occurs. Wild-type GFP, for exam- ple, shows an excitation peak at 395 nm with a shoulder at 475 nm. ...
but the use of FPs in intact animals, such as mice, has been handicapped by poor penetration of excitation light. We now show that a bacteriophytochrome from Deinococcus radiodurans, incorporating biliverdin as the chromophore, can be engineered into monomeric, infrared...
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Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invalua...
Green fluorescent protein (GFP) fluorescence through the Rhodamine channel after excitation using the FITC filter setdoi:10.1016/S1366-2120(08)70081-3MicroscopyYasuyoshi Sakai and Suresh SubramaniTechnical Tips Online
CX-GFP codes for a red-shifted GFP-variant (488 nm excitation, 509 run emission) enabeling visualization of GFP expression by spectroscopy, fluorescence microscopy or FACS-analysis (488 nm excitation, FITC filters).rnGFP expressing cells were subjected to different fixation/staining protocols. ...
(excitation wavelength = 488 nm; emission wavelength = 530 nm; 25 flashes) measured with an EnSpire Multimode Plate Reader (PerkinElmer). In analysis, raw data was corrected for background (subtracting TNG buffer and split GFP S1-10 controls). Sample data is presented as a ...
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses Excitation Laser Blue Laser (488 nm) Application Notes Additional reported app...