Samples were incubated overnight at RT and fluorescence (excitation wavelength = 488 nm; emission wavelength = 530 nm; 25 flashes) measured with an EnSpire Multimode Plate Reader (PerkinElmer). In analysis, raw data was corrected for background (subtracting TNG buffer and split GFP...
The LUYOR-3415RG dual-wavelength fluorescent protein excitation light source (LUYOR, Shanghai, China) was used to screen for transgenic Arabidopsis lines with high EGFP expression levels (excitation and emission wavelengths were 440–460 and 500 nm, respectively). The kanamycin-resistant T1 ...
Photo conversion Any method that enables excitation of, or emission from, GFP at an alternative wavelength may be useful in avoiding areas of the spectrum where autofluores- cence occurs. Wild-type GFP, for exam- ple, shows an excitation peak at 395 nm with a shoulder at 475 nm. ...
emission wavelength max – 502 nm; excitation wavelength max – 482 nm; quantum yield – 0.6; extinction coefficient – 70,000 M-1 cm-1 产品说明书 请联系HonorGene技术支持索取产品说明书。 载体序列 质粒图谱
Briefly, the ratio between absorbance at the excitation wavelength and the integral of emission spectra are measured for each sample and a standard with known quantum yield. Fluorescein and 1-aminoanthracene were used as standards for measurements at excitation wavelengths 450 and 400 nm, respectively...
(Table S4) containing 50 mg/L kanamycin.TheLUYOR-3415RG dual-wavelength fluorescent protein excitation light source(LUYOR, Shanghai, China) was used to screen for transgenic Arabidopsis lines with high EGFP expression levels (excitation and emission wavelengths were 440–460 and 500 nm, ...
The longest-wavelength emission (527 nm) among the GFP-type chromophores is exhibited by yellow fluorescent protein (YFP), which is the result of the T203Y substitution (Ormo et al., 1996). The resulting π-π stacking between the Tyr203 and chromophore phenolic rings and an increased polari...
emission wavelength max – 502 nm; excitation wavelength max – 482 nm; quantum yield – 0.6; extinction coefficient – 70,000 M-1 cm-1 特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途! 关键字: pCDF1-MCS2-EF1-copGFP 载体/pCDF1-MCS2-EF1-copGFP质粒 公司简介 ...
Arabidopsis tissues were observed with Olympus fluorescent microscopy (AH2-RFL) with a filter set providing 455–490 nm excitation and emission above 515 nm. Acknowledgements The first two authors contributed equally to this research. This work was supported by NRICGP-USDA (94-37306-0784), NSF ...
EmissionVisible Excitation Wavelength Range485⁄520 For Use With (Equipment)Fluorescence Microscope, Fluorometer, HTS Reader, High Content Instrument, Flow Cytometer FormLiquid PromoterCMV Quantity1 mL Shipping ConditionWet Ice TechniqueFluorescence Intensity Label TypeFluorescent Dye Product TypeBacMam GFP Tr...