The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. GFPSparkTM is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier ...
虽然许多其他海洋生物也有类似的绿色荧光蛋白,但传统上,绿色荧光蛋白(GFP)指首先从维多利亚多管发光水母中分离的蛋白质。这种蛋白质最早是由下村脩等人在1962年在维多利亚多管发光水母中发现。 11月8日,Scientific Reports 发表了题为:Generation of brilliant green fluorescent petunia plants by using a new and pote...
而虚线则为GFP的发射光谱或荧光光谱,峰值波长约470nm,并在540nm处有一肩峰.这一结果是于1998年,由Tsien3所测得.图2从AequoreaVictoria中所得天然GFP的激发光谱(实线)和荧光发射光谱(虚线)Fluorescenceexcitation(full2line)andemission(dashedline)spectraofnativeGFPfromAequoreavictoriaGFP的晶体结构,如图3所示.它是由...
GFP可以被488 nm激光线激发,并在510 nm处被更佳检测到。Green Fluorescent Protein (GFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. GFP can be excited by the 488 nm laser line and is optimally d...
Excitation max Emission max GFP 395 nm and 475 nm 509 nm EGFP (most common derivative, see below) 488 nm 507 nm For more details see https://www.fpbase.org/protein/avgfp/ and https://www.fpbase.org/protein/egfp/ Which applications can GFP be used for? GFP has been used in...
The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. GFPSparkTM is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier ...
Excitation and emission fluorescence spectra of modified GFP mut2 derivatives. Cells in 20 mM phosphate buffer pH 7.5 (50 碌g/ mL lysozyme) were sonicated and lysates cleared by centrifugation (20,000x g for 30 min). All plasmids carry the GFP-mut2 changes S65A, V68L, and S72A. In ...
Background:Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become...
.Afterthefirstcentrifugation(12000rpmfor15minat4B),thecrudeextractwascentrifugedagain(12000rpmfor20minat4B)tofurtherreduceinsolubleparticleswhichaffectthequantificationoffluorescence.ThentheGFPvalueswerequantifiedbasedonfluorescenceunitsfg-1proteinusingafluorometerrmodelSLM8000)afterexcitationat385nmandmeasuretheemission...
Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invalua...