TABLE 1. Common Sources of Autofluorescence Autofluorescent Source Typical Emission Wavelength (nm) Flavins NADH and NADPH Lipofuscins Advanced glycation end-products (AGEs) Elastin and collagen Lignin Chlorophyll 520 to 560 440 to 470 430 to 670 385 to 450 470 to 520 530 685 (740) Typical ...
北京林业大学在《International Journal of Biological Macromolecules》发表了《Genome-wide identification and functional analysis of silicon transporter family genes in moso bamboo (Phyllostachys edulis)》,文献中使用了LUYOR-3415RG便携式荧光蛋白激发光源用于观察EGFP在拟南芥上的表达。 Silicon (Si) has crucial eff...
Green Fluorescent Protein (GFP) is a 27 kDa protein produced from the jellyfish Aequorea victoria, and is excited by and brightly fluoresces green (emission peak at a wavelength of 509nm) when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence mi...
The LUYOR-3415RG dual-wavelength fluorescent protein excitation light source (LUYOR, Shanghai, China) was used to screen for transgenic Arabidopsis lines with high EGFP expression levels (excitation and emission wavelengths were 440–460 and 500 nm, respectively). The kanamycin-resistant T1 ...
Green Fluorescent Protein (GFP) is a 27 kDa protein produced from the jellyfish Aequorea victoria, and is excited by and brightly fluoresces green (emission peak at a wavelength of 509nm) when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence mi...
Samples were incubated overnight at RT and fluorescence (excitation wavelength = 488 nm; emission wavelength = 530 nm; 25 flashes) measured with an EnSpire Multimode Plate Reader (PerkinElmer). In analysis, raw data was corrected for background (subtracting TNG buffer and split GFP...
Background Green fluorescence protein (GFP) is derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP fluorescence is stable under fixation conditions and suitabl...
emission wavelength max – 502 nm; excitation wavelength max – 482 nm; quantum yield – 0.6; extinction coefficient – 70,000 M-1 cm-1 特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途! 关键字: pCDF1-MCS2-EF1-copGFP 载体/pCDF1-MCS2-EF1-copGFP质粒 公司简介 ...
similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish. The GFP has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible ...
26.9kDa)thatexhibitsbrightgreenfluorescencewhenexposedtobluelight.[1][2]Althoughmanyothermarineorganismshavesimilargreenfluorescentproteins,GFPtraditionallyreferstotheproteinfirstisolatedfromthejellyfishAequoreavictoria.TheGFPfromA.victoriahasamajorexcitationpeakatawavelengthof395nmandaminoroneat475nm.Itsemissionpeakis...