Subsequently, a combination of BiFC coupled with GFP binding peptide (GBP)-nanotrap allowed observation of protein-protein interactions between a co-regulator, HDAC4, and defined BiFC-MEF2 engineered dimers. To determine transactivation properties of defined TF dimers in a cellular system, the Gal4...
For GPCRs, the GFP has been engineered to the N-terminus, the C-terminus, or to the third intracellular loop of a GPCR protein5,7. Nevertheless, because the GPCR termini and intracellular loops can be involved in ligand binding and signal transduction and because a GFP has a molecular ...