DNA methylation, the first layer of epigenetic regulation, is causally implicated in human obesity and T2D through diverse aetiological pathways7,8,9. These pathways include in utero programming of disease risk, long-term effects of diet and lifestyle, mediation of causal genetic variations, age-re...
Genomic DNA is a nucleic acid structure that is comprised of all of an organism's biologically inherited information. Generally long in sequence, gDNA must be sheared to smaller fragment sizes to be used for short-read NGS sequencing.
该产品是从293T细胞中提取的高纯度的基因组DNA提取物,琼脂糖凝胶(0.7%) 电泳检测显示该DNA提取物大小在15Kb以上,且基本无降解,产品最终保存在TE Buffer中,可广泛应用于PCR、酶切、杂交、微阵列分析等分子生物学实验。 产品采用NanoDrop One定量浓度为200 ng/μL。 使用前准备及注意事项 建议-20˚C低温长期保存...
Negative control1835 bp fragment of the human Interferon ß gene, amplified from genomic DNA, purified with Eppendorf Phase Lock GelTM1835 bp fragment from human Interferon ß gene, amplified from genomic DNA, purified without Eppendorf Phase Lock GelTMPlasmid DNA control0.5 µg of DNA size ...
andgel electrophoresis. The Illumina HiSeq systems represent another widely used sequencing by synthesis platform, based on a reversible terminator approach (Fig. 4.3). This technology relies on the attachment of randomly fragmentedgenomic DNAto a flat, transparent surface on a flow cell. These fragm...
Genomic DNA exhibits high heterogeneity in terms of its dynamic within the nucleus, its structure and functional roles. CRISPR-based imaging approaches can image genomic loci in living cells. However, conventional CRISPR-based tools involve expressing constitutively fluorescent proteins, resulting in high...
It is worth mentioning that organellar DNA has been demonstrated not to impact Mash-based phylogeny construction in previous studies, being that their high copy numbers are not represented among low-frequencyk-mers used in Mash phylogeny [63]. Although assembly- or alignment-freek-mer-based methods...
This paper extracts the genomic DNA of the young leaves of pea(Pisum sativum L.)by using an improved CTAB method,and makes the ultraviolet spectrophotometer and agarose gel electrophoresis.The results show that the obtained DNA,which presents better primary structure,higher purity and concentration,co...
Stained colonies were then washed extensively in PBS and water, and quantified on a Gelcount colony counter (Oxford Optronix). SNP arrays Genomic DNA was isolated from ATRX-deficient NHAs at passages 5 and 15. As controls, genomic DNA from ATRX-intact parental NHAs was derived at the start...
德国QIAGEN,货号:19060,Genomic DNA Buffer Set 价格电议 起批量≥1 盒 最小起订1盒 供货总量66盒 发货地址广东省深圳市 建议售价¥0/盒 更新日期2025年01月20日 即时洽谈立即询价查看联系方式 收藏产品发送留言 VIP9年 深圳子科生物科技有限公司