This product is a high purity genomic DNA extract from 293T cells, agarose gel (0.7%) electrophoresis showed that the size of the DNA extract is more than 15Kb, and basically no degradation, the product is ultimately preserved in TE Buffer, which can be widely used in molecular biology expe...
Genomic DNA is subjected to restriction enzyme digestion and size-separated by electrophoresis in an agarose gel. The DNA is transferred to a nylon membrane and analysed using digoxigenin (DIG)-11-dUTP labelled probes. Probes may be reverse transcribed cDNA, single exons, or larger regions of the...
图1.Genomic DNA purified with the Genomic DNA Purification Kit Agarose gel (0.7%) electrophoresis analysis of undigested (odd lane numbers) and EcoRI digested (even lane numbers) DNA samples. M– Lambda DNA/HindIII Marker 1, 2 – human blood ...
To ensure that the results of each gel are reproducible, duplicate gels are prepared. The gels may be analyzed by Southern hybridization, or restriction fragments of known size may be preparatively extracted from agarose plugs. This method can be used to compare the digestion patterns of specific...
图3.Genomic DNA from various sources purified using GeneJET Genomic DNA Purification Kit Genomic DNA purified from various sources using the GeneJET Genomic DNA Purification Kit. 150 ng of isolated genomic DNA from each source was fractionated by agarose gel (0.5%) electrophoresis. ...
图3.Genomic DNA from various sources purified using GeneJET Genomic DNA Purification Kit Genomic DNA purified from various sources using the GeneJET Genomic DNA Purification Kit. 150 ng of isolated genomic DNA from each source was fractionated by agarose gel (0.5%) electrophoresis. ...
Each ladder excised from the plasmids; then the size of the final ladder sequence was confirmed on an agarose gel and quantified by UV fluorometry (Thermofisher Qbuit). In total 14 different DNA ladders were generated. Purified DNA ladders were quantified using the BR dsDNA Qubit Assay on a...
The exponential amplification of the ligated probe sequence generates double stranded DNA products that are unit lengths of the probe [4]. Electrophoretic separation on an agarose gel generates a ladder of DNA bands as seen in Figure 3. Typically, the products ranged in size from 80 bases to ...
Designed to purify high-quality DNA in less than 15 minutes after sample preparation Simple lysis of cells and tissues with Proteinase K without the need for any mechanical lysis Minimal contamination from RNA Reliable performance of the purified DNA in PCR, ...
Total RNA was extracted from each tissue using the RNAprep Pure Plant Kit (Tiangen Biotech, China), and the genomic DNA was removed using RNase-Free DNase I (Takara, Japan). The integrity and quality of RNA were evaluated on a 1.5% agarose gel and NanoDrop 2000 (Thermo Fisher Scientific,...