Colony counting is usually done manually with a pen or with automated gel count systems, such as the Oxford Optronix. These systems provide additional information, such as colony size, are able to handle high plate throughput and offer consistent counting. However, the method is also time...
DNA fragments were analysed by agarose gel electrophoresis. The formation of new cleavage products due to the presence of one or more mismatches was indicated by the presence of additional bands. The relative size of these cleavage products indicated the location of the mismatch or mismatches (...
GEL performed the gene function and pathway analysis. DS conceived and designed the experiments, and prepared the manuscript. All authors read and approved the final manuscript. Xiaogang Cui, Yali Hou contributed equally to this work. Electronic supplementary material 12864_2013_5950_MOESM1_ESM.doc ...
To denature DNA, slides were placed in a horizontal gel electrophoresis tank filled with cold electrophoresis solution (1 mM Na2EDTA, 0.3 M NaOH, pH 13.0) for 40 min at 4 °C. Electrophoresis was performed at 0.7 V/cm, 300 mA for 30 min at 4 °C. Following electrophoresis, slides ...
The daily dose of T-2 toxin was modified to account for the increase in the body weight of animals. The toxin was administered orally in gel capsules, once a day, before morning feeding. At the same time, control group gilts (n = 15) were administered empty gel capsules to maintain ...